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Profiling of rotavirus 3UTR-binding proteins reveals the ATP synthase subunit ATP5B as a host factor that supports late-stage virus replication

文献类型： 外文期刊

作者： Ren, Lili 1 ; Ding, Siyuan 1 ; Song, Yanhua 1 ; Li, Bin 1 ; Ramanathan, Muthukumar 6 ; Co, Julia 2 ; Amieva, Manuel R. 2 ;

作者机构： 1.Stanford Univ, Sch Med, Dept Med, Div Gastroenterol & Hepatol, Stanford, CA 94305 USA

2.Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA

3.Vet Affairs Palo Alto Hlth Care Syst, Palo Alto Vet Inst Res, Palo Alto, CA 94304 USA

4.Nanjing Tech Univ, Sch Pharmaceut Sci, Nanjing 211816, Jiangsu, Peoples R China

5.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Peoples R China

6.Stanford Univ, Sch Med, Program Epithelial Biol, Stanford, CA 94305 USA

关键词： RNA virus; RNA-binding protein; virus assembly; virology; proteomics; ATP synthase; RNA-protein interaction; biotin proximity ligation; nonenveloped double-stranded RNA virus; rotavirus; viral gastroenteritis; virus-host interaction

期刊名称：JOURNAL OF BIOLOGICAL CHEMISTRY （ 影响因子：5.157； 五年影响因子：5.041 ）

ISSN：

年卷期： 2019 年 294 卷 15 期

页码：

收录情况： SCI

摘要： Genome replication and virion assembly of segmented RNA viruses are highly coordinated events, tightly regulated by sequence and structural elements in the UTRs of viral RNA. This process is poorly defined and likely requires the participation of host proteins in concert with viral proteins. In this study, we employed a proteomics-based approach, named RNA-protein interaction detection (RaPID), to comprehensively screen for host proteins that bind to a conserved motif within the rotavirus (RV) 3 terminus. Using this assay, we identified ATP5B, a core subunit of the mitochondrial ATP synthase, as having high affinity to the RV 3UTR consensus sequences. During RV infection, ATP5B bound to the RV 3UTR and co-localized with viral RNA and viroplasm. Functionally, siRNA-mediated genetic depletion of ATP5B or other ATP synthase subunits such as ATP5A1 and ATP5O reduced the production of infectious viral progeny without significant alteration of intracellular viral RNA levels or RNA translation. Chemical inhibition of ATP synthase diminished RV yield in both conventional cell culture and in human intestinal enteroids, indicating that ATP5B positively regulates late-stage RV maturation in primary intestinal epithelial cells. Collectively, our results shed light on the role of host proteins in RV genome assembly and particle formation and identify ATP5B as a novel pro-RV RNA-binding protein, contributing to our understanding of how host ATP synthases may galvanize virus growth and pathogenesis.

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