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Silkworm storage protein Bm30K-19G1 has a certain antifungal effects on Beauveria bassiana

文献类型： 外文期刊

作者： Li, Ruilin 1 ; Hu, Congwu 1 ; Shi, Yali 1 ; Geng, Tao 3 ; Lv, Dingding 4 ; Gao, Kun 1 ; Hou, Chengxiang 1 ; Guo, Xijie 1 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Zhenjiang 212018, Jiangsu, Peoples R China

2.Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Jiangsu, Peoples R China

3.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China

4.Zhenjiang Coll, Zhenjiang 212028, Jiangsu, Peoples R China

关键词： Bombyx mori; Storage protein; Bm30K-19G1; Antifungal function

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）

ISSN： 0022-2011

年卷期： 2019 年 163 卷

页码：

收录情况： SCI

摘要： Storage proteins in the 30 K family are ubiquitous in the hemolymph of insects and play important roles in adult metamorphosis, development, egg formation, carrier transport and even host immunity. Some studies have shown that the 30 K proteins can inhibit apoptosis and have certain antifungal effects. The silkworm protein Bm30K-19G1 is a low molecular weight apolipoprotein that is abundant in hemolymph of fifth instar larvae. Our previous transcriptome sequencing, real-time PCR analysis and proteomic studies showed that the expression level of the 30 K protein gene was significantly up-regulated in the silkworm infected with Beauveria bassiana. In this study, the ORF sequence of Bm30K-19G1 was amplified by PCR. The sequence is 1311 bp in length and encodes a 436 amino acid peptide. Bm30K-19G1 was expressed in all tested tissues of fifth instar larvae, with highest expression in fat body and the lowest expression in the midgut. Bm30K-19G1 protein was successfully expressed in the prokaryotic expression system using pET-28a(+) as vector and E. coli Arctic Express (DE3) as the expression bacterium strain. The expressed recombinant Bm30K-19G1 protein has an inhibitory effect on the conidial germination and hyphal growth of B. bassiana. Bm30K-19G1 also inhibited the growth and reproduction of B. bassiana in vivo; the median lethal time of infected silkworms was postponed by 6.4 h and the time for death of all infected larvae was postponed by 10 h. The results indicated that the silkworm storage protein 30K-19G1 is an antifungal protein against B. bassiana and help to elucidate the molecular mechanism of silkworm resistance against B. bassiana.

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