文献类型： 外文期刊
作者： Liao, Zhihong 1 ; Dai, Zhenkai 1 ; Cai, Chenyu 1 ; Zhang, Xinheng 1 ; Li, Aijun 6 ; Zhang, Huanmin 7 ; Yan, Yiming 1 ; Li 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China
2.Minist Agr, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China
3.Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China
4.Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China
5.Guangdong Prov Anim Virus Vector Vaccine Engn Tec, Guangzhou 510642, Guangdong, Peoples R China
6.Jinan Univ, Coll Sci & Engn, Guangzhou 510632, Guangdong, Peoples R China
7.ARS, Avian Dis & Oncol Lab, USDA, E Lansing, MI 48823 USA
关键词： Atg5; CRISPR/CAS9; DF-1 cells; Apoptosis; Autophagy
期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL （ 影响因子：2.416； 五年影响因子：2.117 ）
ISSN： 1071-2690
年卷期： 2019 年 55 卷 5 期
页码：
收录情况： SCI
摘要： Atg5, as a switch of cell autophagy and apoptosis, plays an important regulatory role in the occurrence and development of autophagy. Atg5 has been reported to involve the autophagy process but little in the apoptotic process. Here, we constructed an Atg5(-/-) DF-1 cell line using the CRISPR/Cas9 assay and confirmed the significant difference in growth kinetics between Atg5(-/-) DF-1 cells and wild-type DF-1 cells. Importantly, we found that Atg5 suppresses the cellular proliferation and induce the apoptosis in DF-1 cells by Hoechst's staining, flow cytometry, and caspase activity assay. All these findings indicated that Atg5 plays an important role in the proliferation of DF-1 cells. On the other hand, we compared the expression of autophagy key proteins LC3 and P62 in Atg5 knockout cells and wild-type cells, and detected the aggregation point distribution of LC3 protein in cells by laser confocal technique; our results showed that Atg5 knockout inhibited autophagy compared with wild-type cells. The present findings further help to resolve the molecular mechanisms regulating Atg5 autophagy and apoptosis.
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