Proteomics analysis reveals heat shock proteins involved in caprine parainfluenza virus type 3 infection
文献类型： 外文期刊
作者： Zhong, Chunyan 1 ; Li, Jizong 1 ; Mao, Li 1 ; Liu, Maojun 1 ; Zhu, Xing 3 ; Li, Wenliang 1 ; Sun, Min 1 ; Ji, Xinqin 3 ; Xia 1 ;
作者机构： 1.Minist Agr, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Jiangsu Acad Agr Sci, Nanjing 210014, Jiangsu, Peoples R China
2.Linyi Univ, Sch Pharm, Linyi 276000, Shandong, Peoples R China
3.Guizhou Univ, Coll Anim Sci, Guiyang 550025, Guizhou, Peoples R China
4.State Key Lab Breeding Base, Key Lab Food Qual & Safety Jiangsu Prov, Nanjing 210014, Jiangsu, Peoples R China
关键词： Caprine parainfluenza virus type 3; Madin-Darby bovine kidney cells; Proteomic analysis; iTRAQ; LC-MS; MS
期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）
ISSN： 1746-6148
年卷期： 2019 年 15 卷
页码：
收录情况： SCI
摘要： BackgroundCaprine parainfluenza virus type 3 (CPIV3) is major pathogen of goat herds causing serious respiratory tract disease and economic losses to the goat industry in China. We analyzed the differential proteomics of CPIV3-infected Madin-Darby bovine kidney (MDBK) cells using quantitative iTRAQ coupled LC-MS/MS. In addition, four DEPs were validated by qRT-PCR and western blot analysis.ResultsQuantitative proteomics analysis revealed 163 differentially expressed proteins (DEPs) between CPIV3-infected and mock-infected groups (p-value <0.05 and fold change >1.2), among which 91 were down-regulated and 72 were up-regulated. Gene ontology (GO) analysis showed that these DEPs were involved in molecular functions, cellular components and biological processes. Biological functions in which the DEPs were involved in included diseases, genetic information processing, metabolism, environmental information processing, cellular processes, and organismal systems. STRING analysis revealed that four heat shock proteins (HSPs) included HSPA5, HSPA1B, HSP90B1 and HSPA6 may be associated with proliferation of CPIV3 in MDBK cells. qRT-PCR and western blot analysis showed that the selected HSPs were identical to the quantitative proteomics data.ConclusionTo our knowledge, this is the first report of the proteomic changes in MDBK cells after CPIV3 infection.
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