Marker-assisted backcrossing to improve seed oleic acid content in four elite and popular peanut (Arachis hypogaea L.) cultivars with high oil content
文献类型： 外文期刊
作者： Huang, Bingyan 1 ; Qi, Feiyan 1 ; Sun, Ziqi 2 ; Miao, Lijuan 1 ; Zhang, Zhongxin 1 ; Liu, Hua 2 ; Fang, Yuanjin 1 ; Dong, 1 ;
作者机构： 1.Henan Acad Agr Sci, Key Lab Oil Crops Huanghuaihai Plains, Ind Crops Res Inst, 116 Huayuan Rd, Zhengzhou 450002, Henan, Peoples R China
2.Henan Prov Key Lab Oil Crops Improvement, 116 Huayuan Rd, Zhengzhou 450002, Henan, Peoples R China
关键词： peanut; Arachis hypogaea; high oleic acid; oil content; marker-assisted backcross selection; single nucleotide polymorphism; genetic background recovery
期刊名称：BREEDING SCIENCE （ 影响因子：2.086； 五年影响因子：2.632 ）
ISSN： 1344-7610
年卷期： 2019 年 69 卷 2 期
页码：
收录情况： SCI
摘要： High oleic acid composition is an important determinant of seed quality in peanut (Arachis hypogaea) in regard to its nutritional benefits for human health and prolonged shelf-life for peanut products. To improve the oleic acid content of popular peanut cultivars in China, four peanut cultivars of different market types were hybridized with high-oleic-acid donors and backcrossed for four generations as recurrent parents using fad2 marker-assisted backcross selection. Seed quality traits in advanced generations derived by selfing were assessed using near-infrared reflectance spectroscopy for detection of oleic acid and Kompetitive allele-specific PCR (KASP) screening of fad2 mutant markers. Twenty-four high-oleic-acid lines of BC4F4 and BC4F5 populations, with morphological features and agronomic traits similar to those of the recurrent parents, were obtained within 5 years. The genetic backgrounds of BC4F5 lines were estimated using the KASP assay, which revealed the genetic background recovery rate was 79.49%-92.31%. The superior lines raised are undergoing a multi-location test for cultivar registration and release. To our knowledge, this is the first application of single nucleotide polymorphism markers based on the high-throughput and cost-effective KASP assay for detection of fad2 mutations and genetic background evaluation in a peanut breeding program.
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