您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Virtual Screening-Based Peptides Targeting Spike Protein to Inhibit Porcine Epidemic Diarrhea Virus (PEDV) Infection

文献类型： 外文期刊

作者： Xu, Qian 1 ; Wang, Fangyu 2 ; Jiao, Wenqiang 2 ; Zhang, Mengting 1 ; Xing, Guangxu 2 ; Feng, Hua 2 ; Sun, Xuefeng 2 ; Hu, Man 2 ; Zhang, Gaiping 1 ;

作者机构： 1.Northwest A&F Univ, Coll Vet Med, Dept Prevent Vet Med, Xianyang 712100, Peoples R China

2.Henan Acad Agr Sci, Key Lab Anim Immunol, 116 Huayuan Rd, Zhengzhou 450002, Peoples R China

3.Longhu Modern Immunol Lab, Zhengzhou 450046, Peoples R China

4.Peking Univ, Sch Adv Agr Sci, Beijing 100871, Peoples R China

5.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Peoples R China

关键词： porcine epidemic diarrhea virus; S1 C-terminal domain (CTD) protein; virtual screening; SPR; antiviral peptides; qRT-PCR; indirect immunofluorescence

期刊名称：VIRUSES-BASEL （ 影响因子：4.7； 五年影响因子：4.8 ）

ISSN：

年卷期： 2023 年 15 卷 2 期

页码：

收录情况： SCI

摘要： Due to the rapid mutation of porcine epidemic diarrhea virus (PEDV), existing vaccines cannot provide sufficient immune protection for pigs. Therefore, it is urgent to design the affinity peptides for the prevention and control of this disease. In this study, we made use of a molecular docking technology for virtual screening of affinity peptides that specifically recognized the PEDV S1 C-terminal domain (CTD) protein for the first time. Experimentally, the affinity, cross-reactivity and sensitivity of the peptides were identified by an enzyme-linked immunosorbent assay (ELISA) and a surface plasmon resonance (SPR) test, separately. Subsequently, Cell Counting Kit-8 (CCK-8), quantitative real-time PCR (qRT-PCR), Western blot and indirect immunofluorescence were used to further study the antiviral effect of different concentrations of peptide 110766 in PEDV. Our results showed that the P/N value of peptide 110766 at 450 nm reached 167, with a K-D value of 216 nM. The cytotoxic test indicated that peptide 110766 was not toxic to vero cells. Results of the absolute quantitative PCR revealed that different concentrations (3.125 mu M, 6.25 mu M, 12.5 mu M, 25 mu M, 50 mu M, 100 mu M, 200 mu M) of peptide 110766 could significantly reduce the viral load of PEDV compared with the virus group (p < 0.0001). Similarly, results of Western blot and indirect immunofluorescence also suggested that the antiviral effect of peptide 110766 at 3.125 is still significant. Based on the above research, high-affinity peptide 110766 binding to the PEDV S1-CTD protein was attained by a molecular docking technology. Therefore, designing, screening, and identifying affinity peptides can provide a new method for the development of antiviral drugs for PEDV.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台