文献类型： 外文期刊

作者： Du, Rongqi ¹ ; He, Jinling ¹ ; Meng, Jiali ² ; Zhang, Dongchao ¹ ; Li, Danruo ¹ ; Wang, Hui ¹ ; Fan, Aili ⁵ ; Xu, Gang ¹ ; Ma, Shuhui ¹ ; Zuo, Zonghui ¹ ; Song, Qiqi ¹ ; Jin, Tianming ³ ;

作者机构： 1.Tianjin Agr Univ, Coll Anim Sci & Vet Med, Tianjin Key Lab Agr Anim Breeding & Hlth Husb, Tianjin 300392, Peoples R China

2.Capital Med Univ, Beijing Anzhen Hosp, Beijing Inst Heart Lung & Blood Vessel Dis, Beijing 100029, Peoples R China

3.Tianjin Acad Agr Sci, Inst Anim Sci & Vet, Tianjin Key Lab Anim Mol Breeding & Biotechnol, Tianjin 300381, Peoples R China

4.Tianjin Agr Univ, Coll Anim Sci & Vet Med, Tianjin Engn Technol Ctr Livestock Pathogen Detect, Tianjin 300392, Peoples R China

5.Hengnuoyou Tianjin Biotechnol Co Ltd, Tianjin 301600, Peoples R China

6.Tianjin Agr Univ, Minist Agr & Rural Afairs, Key Lab Smart Breeding Coconstruct Minist & Prov, Tianjin 300392, Peoples R China

关键词： Toxoplasma gondii; DNA vaccine; Rhoptry proteins; Protective immunity

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.0； 五年影响因子：2.1 ）

ISSN： 0932-0113

年卷期： 2024 年 123 卷 12 期

页码：

收录情况： SCI

摘要： Toxoplasmosis is a foodborne zoonotic parasitic disease caused by Toxoplasma gondii, which seriously threatens to human health and causes economic losses. At present, there is no effective vaccine strategy for the prevention and control of toxoplasmosis. T. gondii rhoptry proteins (ROPs) are important proteins secreted by the parasite during the early stage of invasion into host cells. In this study, we constructed six individual plasmids (pVAX1-ROP2, pVAX1-ROP5, pVAX1-ROP9, pVAX1-ROP16, pVAX1-ROP17, and pVAX1-ROP18) encoding T. gondii rhoptry proteins and then used an equimolar amount of each as a vaccine cocktail. Following booster immunization, serum antibody levels, splenic lymphocyte proliferation, cytokine production, and survival time after infection with T. gondii RH strain were measured in immunized mice. The results showed that the mice immunized with the DNA vaccine cocktail developed a higher level of the specific anti-T. gondii IgG in serum and the cytokines such as IFN-gamma, IL-2, IL-12, and IL-4 (P < 0.01). The stimulation index (SI) of spleen lymphocytes (P < 0.01), the frequencies of CD4+ T lymphocytes (P < 0.01), and the ratio of CD4+/CD8+ T lymphocytes (P < 0.05 or P < 0.01) in the vaccine-immunized mice were significantly increased compared to the control group. After challenge with the virulent T. gondii RH strain tachyzoites, the survival time of mice in the DNA vaccine cocktail group (18.1 +/- 1.81 d) was significantly longer (P < 0.01) than that in the control group (8.4 +/- 1.02 or 7.9 +/- 0.83 d). The results indicated that the DNA vaccine cocktail could elicit strong humoral and cellular immune responses in mice and could also improve the resistance of mice to acute T. gondii infection.