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GhEXL3 participates in brassinosteroids regulation of fiber elongation in Gossypium hirsutum

文献类型： 外文期刊

作者： Zhang, Changsheng 1 ; Liu, Zhao 1 ; Shu, Sheng 1 ; Li, Xinyang 2 ; Li, Yujun 2 ; Liu, Le 2 ; Liu, Li 4 ; Wang, Xuwen 4 ; Li, Fuguang 1 ; Qanmber, Ghulam 1 ; Yang, Zuoren 1 ;

作者机构： 1.Zhengzhou Univ, Sch Agr Sci, State Key Lab Cotton Biobreeding & Integrated Util, Zhengzhou Res Base, Zhengzhou, Peoples R China

2.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biobreeding & Integrated Util, Anyang, Peoples R China

3.Chinese Acad Agr Sci, Western Agr Res Ctr, Changji, Peoples R China

4.Xinjiang Acad Agr & Reclamat Sci, Cotton Res Inst, Northwest Inland Reg Key Lab Cotton Biol & Genet B, Shihezi, Peoples R China

关键词： cotton; fiber; brassinosteroids; GhEXL3; GhBES1.4; cell elongation

期刊名称：PLANT JOURNAL （ 影响因子：6.2； 五年影响因子：7.1 ）

ISSN： 0960-7412

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Cotton fiber (Gossypium hirsutum) serves as an ideal model for investigating the molecular mechanisms of plant cell elongation at the single-cell level. Brassinosteroids (BRs) play a crucial role in regulating plant growth and development. However, the mechanism by which BR influences cotton fiber elongation remains incompletely understood. In this study, we identified EXORDIUM-like (GhEXL3) through transcriptome analysis of fibers from BR-deficient cotton mutant pagoda 1 (pag1) and BRI1-EMS-SUPPRESSOR 1 (GhBES1.4, encoding a central transcription factor of BR signaling) overexpression cotton lines. Knockout of GhEXL3 using CRISPR/Cas9 was found to impede cotton fiber elongation, while its overexpression promoted fiber elongation, suggesting a positive regulatory function for GhEXL3 in fiber elongation. Furthermore, in vitro ovule culture experiments revealed that the overexpression of GhEXL3 partially counteracted the inhibitory effects of brassinazole (BRZ) on cotton fiber elongation, providing additional evidence of GhEXL3 involvement in BR signaling pathways. Moreover, our findings demonstrate that GhBES1.4 directly binds to the E-box (CACGTG) motif in the GhEXL3 promoter region and enhances its transcription. RNA-seq analysis revealed that overexpression of GhEXL3 upregulated the expression of EXPs, XTHs, and other genes associated with fiber cell elongation. Overall, our study contributes to understanding the mechanism by which BR regulates the elongation of cotton fibers through the direct modulation of GhEXL3 expression by GhBES1.4.

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