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Characterization and fine mapping of a yellow leaf gene regulating chlorophyll biosynthesis and chloroplast development in cotton (Gossypium arboreum)

文献类型： 外文期刊

作者： Fan, Liqiang 1 ; Hou, Yan 1 ; Zheng, Lei 1 ; Shi, Huiyun 1 ; Liu, Zhao 2 ; Wang, Yuxuan 1 ; Li, Shengdong 2 ; Liu, Le 2 ; Guo, Mengzhen 2 ; Yang, Zuoren 1 ; Liu, Ji 1 ;

作者机构： 1.Chinese Acad Agr Sci, State Key Lab Cotton Biol, Inst Cotton Res, Anyang 455000, Peoples R China

2.Zhengzhou Univ, Sch Agr Sci, State Key Lab Cotton Biol, Zhengzhou Res Base, Zhengzhou 450001, Peoples R China

3.Chinese Acad Agr Sci CAAS, Inst Crop Sci, Key Lab Biol & Genet Improvement Triticeae Crops, Minist Agr, Beijing 100081, Peoples R China

4.Xinjiang Acad Agr & Reclamat Sci, Cotton Res Inst, Key Lab China Northwestern Inland Reg, Minist Agr & Rural Affairs, Shihezi 832003, Peoples R China

5.Chinese Acad Agr Sci, Western Agr Res Ctr, Changji 831100, Peoples R China

关键词： Gossypium arboreum; EMS; MutMap; GaCHLH; Chlorophyll biosynthesis

期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）

ISSN： 0378-1119

年卷期： 2023 年 885 卷

页码：

收录情况： SCI

摘要： Chlorophyll biosynthesis and chloroplast development are essential for photosynthesis and plant growth. Gossypium arboreum, a valuable source of genetic variation for cotton improvement, remains poorly studied for the mechanisms regulating chlorophyll biosynthesis and chloroplast development. Here we created a G. arboreum etiolated leaf and stuntedness (els) mutant that displayed a distinct yellow color of leaves, bracts and stems throughout the whole growth, where chlorophyll accumulation in leaves was reduced and chloroplast development was delayed. The GaCHLH gene, which encodes the H subunit of magnesium chelatase (Mg-chelatase), was screened by MutMap and KASP analysis. Compared to GaCHLH, the gene Gachlh of the mutant had a single nucleotide transition (G to A) at 1549 bp, which causes the substitution of a glycine (G) by a serine (S) at the 517th amino acid, resulting in an abnormal secondary structure of the Gachlh protein. GaCHLH-silenced SXY1 and ZM24 plants exhibited a lower GaCHLH expression level, a lower chlorophyll content, and the yellow-leaf phenotype. Gachlh expression affected the expression of key genes in the tetrapyrrole pathway. GaCHLH and Gachlh were located in the chloroplasts and that alteration of the mutation site did not affect the final target position. The BiFC assay result indicated that Gachlh could not bind to GaCHLD properly, which prevented the assembly of Mg-chelatase and thus led to the failure of chlorophyll synthesis. In this study, the Gachlh gene of G. arboreum els was finely localized and identified for the first time, providing new insights into the chlorophyll biosynthesis pathway in cotton.

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