您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Apoptosis in spotted sea bass brain and astroglia-like cell line implies a possible pathogenesis of Lateolabrax maculatus iridovirus infection

文献类型： 外文期刊

作者： Xu, Jingjing 1 ; Ma, Yanping 1 ; Hao, Le 1 ; Liu, Zhenxing 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Peoples R China

2.Key Lab Livestock Dis Prevent Guangdong Prov, Guangzhou 510640, Peoples R China

关键词： Lateolabrax maculatus; Iridovirus; Apoptosis; Astroglia-like cell line; Mitochondria; Bax

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.5； 五年影响因子：3.1 ）

ISSN： 0920-1742

年卷期： 2025 年 51 卷 4 期

页码：

收录情况： SCI

摘要： Brain undergoing apoptosis often results in serious dysfunction, especially when subjected to viral infection. Our previous findings revealed that Lateolabrax maculatus iridovirus (LMIV), genetically closely related to Red Sea Bream Iridovirus (RSIV), was able to invade the brain tissue of L. maculatus. The analysis of consequent brain injury may bring us explanations for the pathogenesis of LMIV infection. In the present study, viral loads in different brain segments (telencephalon, optic tectum, cerebellum, hypothalamus, medulla oblongata, optic nerve) of L. maculatus were measured by qPCR. Then, injury and apoptosis in abovementioned brain divisions were compared by HE staining and TUNEL staining in histological slides, respectively. In vitro assays were further performed using an astroglia-like cell line, LMB-L, developed from brain of L. maculatus. In addition, LMIV-induced apoptotic LMB-L cells were examined utilizing classic methods such as Annexin V/PI staining, JC-1 staining, detection of caspase activity as well as qRT-PCR. We found that LMIV exhibited tropism for different brain regions, of which cerebellum and optic nerve containing the highest viral loads, varying from 188-fold to 115-fold compared with medulla oblongata. Correspondingly, LMIV infection led to serious apoptosis in cerebellum, optic nerve, and optic tectum showing plenty of TUNEL-positive cells. This phenomenon seemed to be congruent with obvious morphological changes in cerebellum and optic nerve with decrease of cells and loose tissue. The apoptosis rates of virus-infected LMB-L cells dramatically increased from 13.43 to 42.32%. Apoptosis in LMB-L cells coincided with depolarization of mitochondrial membrane potential, significant activation of caspase 9, upregulation of the p53 (22.3-fold), Bax (62-fold), and downregulation of Mcl-1, which suggested that the apoptosis was probably mediated by mitochondrial signaling pathway. Moreover, it was also observed that transcription of Fas gene and activity of caspase 8 were evoked by virus infection in LMB-L cells. Thus, death receptor pathway may also be involved in the LMIV-induced apoptosis. Our findings provide new insights into the understanding about the pathogenesis of LMIV infection and apoptosis mechanisms mediated by virus.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台