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Unveiling of brain transcriptome of masked palm civet (Paguma larvata) with chronic infection of Toxoplasma gondii

文献类型： 外文期刊

作者： Yuan, Hao 1 ; Zhang, Xiu-Xiang 8 ; Yang, Zi-Peng 2 ; Wang, Xiao-Hu 5 ; Mahmmod, Yasser S. 6 ; Zhang, Pian 2 ; Yan, Zi-Jing 2 ; Wang, Yan-Yun 2 ; Ren, Zhao-Wen 2 ; Guo, Qing-Yong 1 ; Yuan, Zi-Guo 2 ;

作者机构： 1.Xinjiang Agr Univ, Coll Vet Med, Urumqi 830052, Xinjiang, Peoples R China

2.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China

3.Key Lab Zoonosis Prevent & Control Guangdong Prov, Guangzhou 510642, Peoples R China

4.South China Agr Univ, Minist Agr & Rural Affairs, Key Lab Zoonosis, Guangzhou 510642, Guangdong, Peoples R China

5.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Guangdong, Peoples R China

6.Zagazig Univ, Fac Vet Med, Dept Anim Med, Infect Dis, Zagazig 44511, Sharika, Egypt

7.Higher Coll Technol, Fac Hlth Sci, Vet Sci Div, Abu Dhabi 17155, U Arab Emirates

8.South China Agr Univ, Coll Agr, Guangzhou 510642, Guangdong, Peoples R China

关键词： Toxoplasma gondii; Masked palm civet; Gene expression; RNA-Seq

期刊名称：PARASITES & VECTORS （ 影响因子：4.047； 五年影响因子：4.223 ）

ISSN： 1756-3305

年卷期： 2022 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Background: The aim of this study was to gain an understanding of the transcriptomic changes that occur in a wild species when infected with Toxoplasma gondii. The masked palm civet, an artifically domesticated animal, was used as the model of a wild species. Transcriptome analysis was used to study alterations in gene expression in the domesticated masked palm civet after chronic infection with T. gondii. Methods: Masked palm civets were infected with 10(5) T. gondii cysts and their brain tissue collected after 4 months of infection. RNA sequencing (RNA-Seq) was used to gain insight into the spectrum of genes that were differentially expressed due to infection. Quantitative reverse-transcription PCR (qRT-PCR) was also used to validate the level of expression of a set of differentially expressed genes (DEGs) obtained by sequencing. Results: DEGs were screened from the sequencing results and analyzed. A total of 2808 DEGs were detected, of which 860 were upregulated and 1948 were downregulated. RNA-Seq results were confirmed by qRT-PCR. DEGs were mainly enriched in cellular process and metabolic process based on gene ontology enrichment analysis. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that transcriptional changes in the brain of infected masked palm civets evolved over the course of infection and that DEGs were mainly enriched in the signal transduction, immune system processes, transport and catabolic pathways. Finally, 10 essential driving genes were identified from the immune signaling pathway. Conclusions: This study revealed novel host genes which may provide target genes for the development of new therapeutics and detection methods for T. gondii infection in wild animals.

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