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Marek's disease virus encoded miR-M6 and miR-M10 are dispensable for virus replication and pathogenesis in chickens

文献类型： 外文期刊

作者： Yang, Shuaikang 1 ; Liao, Yifei 3 ; Zhang, Shuai 1 ; Lu, Wenlong 1 ; Jin, Jiaxin 1 ; Teng, Man 4 ; Chai, Shujun 4 ; Luo, 1 ;

作者机构： 1.Henan Agr Univ, Coll Vet Med, Zhengzhou 450002, Henan, Peoples R China

2.Henan Agr Univ, Coll Vet Med, Int Joint Res Ctr Natl Anim Immunol, Zhengzhou 450002, Henan, Peoples R China

3.Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Div Infect Dis, Boston, MA 02115 USA

4.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr & Rural Affairs, Zhengzhou 450002, Henan, Peoples R China

5.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China

6.Henan Acad Agr Sci, UK China Ctr Excellence Res Avian Dis, Zhengzhou 450002, Henan, Peoples R China

7.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

关键词： MiRNAs; miR-M6; miR-M10; Virus replication; Pathogenesis

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2021 年 262 卷

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) are a class of approximately 22 nucleotides long non-coding RNAs, and virus-encoded miRNAs play an important role in pathogenesis. Marek's disease virus (MDV) is an oncogenic avian alphaherpesvirus that causes immunosuppression and tumors in its natural host, chicken. In the MDV genome, 14 miRNA precursors and 26 mature miRNAs were identified, thus MDV has been used as a model to study the function of viral miRNAs in vivo. Recently, a cluster of miRNAs encoded by MDV, Cluster 3 miRNAs (miR-M8M10), has been shown to restrict early cytolytic replication and pathogenesis of MDV. In this study, we further analyzed the role of miR-M6 and miR-M10, members of cluster miR-M8-M10, in MDV replication and pathogenicity. We found that, compared to parental MDV, deletion of miR-M6-5p significantly enhanced the replication of MDV in cell culture, but not in chickens. The replication of miR-M6-5p deletion MDV was restored once the deleted sequences were re-inserted. Our results also showed that deletion of miR-M10-5p did not affect the replication of MDV in vitro and in vivo. In addition, our animal study results showed that deletion of miRM6- 5p or miR-M10-5p did not alter the pathogenesis of MDV. In conclusion, our study shows that both miR-M6 and miR-M10 are dispensable for MDV replication and pathogenesis in chickens, while also suggests a repressive role of miR-M6 in MDV replication in cell culture.

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