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Immunoprotective Effect of Coptis chinensis-Supplemented Diet on Streptococcus agalactiae Infection in Tilapia

文献类型： 外文期刊

作者： Guo, Ruijie 1 ; Yu, Kai 1 ; Huang, Kai 1 ; Lin, Qiang 3 ; Liu, Ting 1 ;

作者机构： 1.Guangxi Univ, Coll Anim Sci & Technol, Nanning 530004, Peoples R China

2.Nanning Current Sci Biotechnol Co Ltd, Dept Om Technol, Nanning 530005, Peoples R China

3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Peoples R China

关键词： tilapia; Streptococcus agalactiae; Coptis chinensis; proteomic; antimicrobial peptide; immune factor; antioxidant enzyme

期刊名称：FISHES （ 影响因子：2.3； 五年影响因子：2.4 ）

ISSN：

年卷期： 2023 年 8 卷 7 期

页码：

收录情况： SCI

摘要： This study aimed to investigate the immunoprotective effect of Coptis chinensis (CC) on Streptococcus agalactiae (SA) infection in tilapia. Experimental fish were randomly divided into two groups feeding on a normal diet (ND) and a CC-supplemented diet (CCD) for 2 weeks and then injected with SA. After the inoculation experiment, the ND and CCD groups were named PI_ND and PI_CCD, respectively. CCD increased superoxide dismutase (SOD) activity and decreased malondialdehyde (MDA) activity significantly before and after infection. Immunological assays revealed that the serum interleukin-1 & beta; (IL-1 & beta;), complement 3 (C3), immunoglobulin M (IgM), Interferon-gamma (IFN-& gamma;), and tumor necrosis factor-& alpha; (TNF-& alpha;) levels in the CCD group were significantly higher than in the ND group both before and after infection. In addition, proteomics analysis of liver tissue identified 62 differentially expressed proteins (DEPs) in CCD vs. ND, and 36 DEPs in the PI_CCD vs. the PI_ND groups. Furthermore, 80 specific upregulated proteins and 49 specific downregulated proteins were screened in the CCD group. The specific upregulated proteins included important antimicrobial enzymes such as lysozymes and cathepsin D, and antimicrobial peptides such as septins, granulin, and grancalcin, involving multiple KEGG brite categories such as enzymes, exosomes, membrane transport, and proteolipid proteins. Furthermore, specific downregulated proteins were enriched in glycolysis/gluconeogenesis and TCA cycle pathways. In conclusion, CC supplementation effectively enhances the ability of tilapia to resist SA infection by modulating various antioxidant enzymes, immune factors, antimicrobial enzymes, and antimicrobial peptides, and by moderately inhibiting central carbon metabolism. These findings provide a basis for replacing antibiotics with environmentally-friendly functional aquatic feeds to control bacterial diseases.

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