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Metabolic engineering for the biosynthesis of bis-indolylquinone terrequinone A in Escherichia coli from L-tryptophan and prenol

文献类型： 外文期刊

作者： Wang, Lijuan 1 ; Deng, Yongdong 1 ; Peng, Rihe 1 ; Gao, Jianjie 1 ; Li, Zhenjun 1 ; Zhang, Wenhui 1 ; Xu, Jing 1 ; Wang, Bo 1 ; Wang, Yu 1 ; Han, Hongjuan 1 ; Fu, Xiaoyan 1 ; Tian, Yongsheng 1 ; Yao, Quanhong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Safety Assessment Environm Agr Genet Modif, Shanghai, Peoples R China

关键词： Biosynthesis; Bis-indolylquinone; Terrequinone A; Dimethylallyl diphosphate (DMAPP); L-tryptophan (L-Trp); Escherichia coli

期刊名称：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS

ISSN：

年卷期： 2023 年 16 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundTerrequinone A is a bis-indolylquinone natural product with antitumor activity. Due to its unique asymmetric quinone core structure and multiple functional groups, biosynthesis is more efficient and environmentally friendly than traditional chemical synthesis. Currently, most bis-indolylquinones are obtained by direct extraction from fungi or by chemical synthesis. By focusing on the biosynthesis of terrequinone A, we hope to explore the way to synthesize bis-indolylquinones de novo using Escherichia coli as a cell factory.ResultsIn this study, a terrequinone A synthesis pathway containing the tdiA-tdiE genes was constructed into Escherichia coli and activated by a phosphopantetheinyl transferase gene sfp, enabling the strain to synthesize 1.54 mg/L of terrequinone A. Subsequently, a two-step isopentenol utilization pathway was introduced to enhance the supply of endogenous dimethylallyl diphosphate (DMAPP) in E. coli, increasing the level of terrequinone A to 20.1 mg/L. By adjusting the L-tryptophan (L-Trp)/prenol ratio, the major product could be changed from ochrindole D to terrequinone A, and the content of terrequinone A reached the highest 106.3 mg/L under the optimized culture conditions. Metabolic analysis of L-Trp indicated that the conversion of large amounts of L-Trp to indole was an important factor preventing the further improvement of terrequinone A yield.ConclusionsA comprehensive approach was adopted and terrequinone A was successfully synthesized from low-cost L-Trp and prenol in E. coli. This study provides a metabolic engineering strategy for the efficient synthesis of terrequinone A and other similar bis-indolylquinones with asymmetric quinone cores. In addition, this is the first report on the de novo biosyhthesis of terrequinone A in an engineered strain.

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