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The Xanthomonas type III effector XopAP prevents stomatal closure by interfering with vacuolar acidification

文献类型： 外文期刊

作者： Liu, Longyu 1 ; Li, Ying 1 ; Xu, Zhengyin 1 ; Chen, Huan 2 ; Zhang, Jingyi 2 ; Manion, Brittany 2 ; Liu, Fengquan 3 ; Zou, Lifang 1 ; Fu, Zheng Qing 2 ; Chen, Gongyou 1 ;

作者机构： 1.Shanghai Jiao Tong Univ, Shanghai Collaborat Innovat Ctr Agriseeds, Sch Agr & Biol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China

2.Univ South Carolina, Dept Biol Sci, Columbia, SC 29208 USA

3.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China

关键词： PtdIns(3; 5)P-2; stomatal closure; type III effector; vacuole acidification; Xanthomonas; XopAP

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：9.106； 五年影响因子：8.241 ）

ISSN： 1672-9072

年卷期： 2022 年 64 卷 10 期

页码：

收录情况： SCI

摘要： Plant stomata close rapidly in response to a rise in the plant hormone abscisic acid (ABA) or salicylic acid (SA) and after recognition of pathogen-associated molecular patterns (PAMPs). Stomatal closure is the result of vacuolar convolution, ion efflux, and changes in turgor pressure in guard cells. Phytopathogenic bacteria secrete type III effectors (T3Es) that interfere with plant defense mechanisms, causing severe plant disease symptoms. Here, we show that the virulence and infection of Xanthomonas oryzae pv. oryzicola (Xoc), which is the causal agent of rice bacterial leaf streak disease, drastically increased in transgenic rice (Oryza sativa L.) plants overexpressing the Xoc T3E gene XopAP, which encodes a protein annotated as a lipase. We discovered that XopAP binds to phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P-2), a membrane phospholipid that functions in pH control in lysosomes, membrane dynamics, and protein trafficking. XopAP inhibited the acidification of vacuoles by competing with vacuolar H+-pyrophosphatase (V-PPase) for binding to PtdIns(3,5)P-2, leading to stomatal opening. Transgenic rice overexpressing XopAP also showed inhibition of stomatal closure when challenged by Xoc infection and treatment with the PAMP flg22. Moreover, XopAP suppressed flg22-induced gene expression, reactive oxygen species burst and callose deposition in host plants, demonstrating that XopAP subverts PAMP-triggered immunity during Xoc infection. Taken together, these findings demonstrate that XopAP overcomes stomatal immunity in plants by binding to lipids.

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