文献类型： 外文期刊
作者： Wang, Lijuan 1 ; Peng, Rihe 1 ; Tian, Yongsheng 1 ; Xu, Jing 1 ; Wang, Bo 1 ; Han, Hongjuan 1 ; Fu, Xiaoyan 1 ; Gao, Jianjie 1 ; Yao, Quanhong 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai, Peoples R China
关键词： Multi-monocistronic; 4-Fluorophenol; beta-Ketoadipate; Degradation; 4-Substituted phenol
期刊名称：AMB EXPRESS （ 影响因子：4.126； 五年影响因子：4.245 ）
ISSN： 2191-0855
年卷期： 2022 年 12 卷 1 期
页码：
收录情况： SCI
摘要： As a kind of refractory organic pollutant, 4-fluorophenol (4-FP) can be degraded by only a few microorganisms with low efficiency because of the great electron-withdrawing ability of fluorine atoms. So it is necessary to artificially construct engineered strain to improve the degradation efficiency and meet the requirements of pollutant degradation. In this study, four genes (fpdA2, fpdB, fpdC, and fpdD) for 4-FP degradation from Arthrobacter sp. strain IF1 were optimized and synthesized and then reconstructed into Escherichia coli by a multi-monocistronic vector to obtain recombinant BL-fpd that could degrade 4-FP efficiently. Under optimized induction conditions (inducing the strain by 2 g/L L-arabinose and 1 mM IPTG at 37 degrees C), BL-fpd could completely degrade 2 mM 4-FP, 4-chlorophenol, 4-bromophenol, and 4-nitrophenol into beta-ketoadipate, which could be further metabolized by the bacteria. FpdA2 showed the highest activity towards 4-bromophenol. The strain could completely degrade 1 mM 4-FP in industrial wastewater within 3 h. This study provided a promising strain for the degradation of 4-FP and some other 4-substituted phenols. The construction technologies of multi-monocistronic expression vector may also be used to construct other organic pollutants degrading bacteria.
- 相关文献
作者其他论文 更多>>
-
Synthesis of betanin by expression of the core betalain biosynthetic pathway in carrot
作者：Wang, Bo;Wang, Yahui;Deng, Yuanjie;Xiong, Aisheng;Wang, Bo;Yao, Quanhong
关键词：Daucus carota L.; Betanin; Biosynthesis; Metabolic engineering; Bioforti fication
-
The complete degradation of 1,2-dichloroethane in Escherichia coli by metabolic engineering
作者：Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong;Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong
关键词：Bioremediation; Engineered bacteria; Environmental pollution; Metabolic engineering; Synthetic biology
-
Ectopic expression of an Old Yellow Enzyme (OYE3) gene from Saccharomyces cerevisiae increases the tolerance and phytoremediation of 2-nitroaniline in rice
作者：Li, Zhenjun;Gao, Jianjie;Wang, Bo;Zhang, Hao;Tian, Yongsheng;Peng, Rihe;Yao, Quanhong
关键词：2-nitroaniline; Old Yellow Enzyme; Phytoremediation; Reactive oxygen species; Saccharomyces cerevisiae
-
Biosynthesis of melatonin from
l -tryptophan by an engineered microbial cell factory作者：Wang, Lijuan;Deng, Yongdong;Gao, Jianjie;Wang, Bo;Han, Hongjuan;Li, Zhenjun;Zhang, Wenhui;Wang, Yu;Fu, Xiaoyan;Peng, Rihe;Yao, Quanhong;Tian, Yongsheng;Xu, Jing;Wang, Lijuan;Deng, Yongdong;Peng, Rihe;Yao, Quanhong;Tian, Yongsheng;Xu, Jing
关键词：Biosynthesis; Melatonin;
l -Tryptophan (l -Trp); Escherichia coli; Metabolic engineering -
Effects of Light Intensity on Growth and Quality of Lettuce and Spinach Cultivars in a Plant Factory
作者：Miao, Chen;Wang, Hong;Zhang, Yongxue;Cui, Jiawei;Zhang, Hongmei;Jin, Haijun;Lu, Panling;He, Lizhong;Zhou, Qiang;Yu, Jizhu;Ding, Xiaotao;Yang, Shaojun;Xu, Jing
关键词：indoor cultivation; LED light intensity; plant development; photosynthesis; nutrient content; tipburn
-
Metabolic engineering of Escherichia coli for 2,4-dinitrotoluene degradation
作者：Zhang, Wen-Hui;Deng, Yong-Dong;Zuo, Zhi-Hao;Tian, Yong-Sheng;Xu, Jing;Wang, Bo;Wang, Li-Juan;Han, Hong-Juan;Li, Zhen-Jun;Wang, Yu;Yao, Quan-Hong;Gao, Jian-Jie;Fu, Xiao-Yan;Peng, Ri-He;Zhang, Wen-Hui;Deng, Yong-Dong;Zuo, Zhi-Hao;Tian, Yong-Sheng;Xu, Jing;Wang, Bo;Wang, Li-Juan;Han, Hong-Juan;Li, Zhen-Jun;Wang, Yu;Yao, Quan-Hong;Gao, Jian-Jie;Fu, Xiao-Yan;Peng, Ri-He;Chen, Zhi-Feng
关键词：4-Dinitrotoluene; Re-synthesize gene; Complete degradation; Bioremediation; Escherichia coli
-
Creation of Environmentally Friendly Super "Dinitrotoluene Scavenger" Plants
作者：Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Zhu, Bo
关键词：2,4-dinitrotoluene (2,4-DNT); carbon neutrality; complete degradation; rapid phytoremediation; sustainable development goals
