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Metabolic engineering of Escherichia coli for efficient degradation of 4-fluorophenol

文献类型： 外文期刊

作者： Wang, Lijuan 1 ; Peng, Rihe 1 ; Tian, Yongsheng 1 ; Xu, Jing 1 ; Wang, Bo 1 ; Han, Hongjuan 1 ; Fu, Xiaoyan 1 ; Gao, Jianjie 1 ; Yao, Quanhong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai, Peoples R China

关键词： Multi-monocistronic; 4-Fluorophenol; beta-Ketoadipate; Degradation; 4-Substituted phenol

期刊名称：AMB EXPRESS （ 影响因子：4.126； 五年影响因子：4.245 ）

ISSN： 2191-0855

年卷期： 2022 年 12 卷 1 期

页码：

收录情况： SCI

摘要： As a kind of refractory organic pollutant, 4-fluorophenol (4-FP) can be degraded by only a few microorganisms with low efficiency because of the great electron-withdrawing ability of fluorine atoms. So it is necessary to artificially construct engineered strain to improve the degradation efficiency and meet the requirements of pollutant degradation. In this study, four genes (fpdA2, fpdB, fpdC, and fpdD) for 4-FP degradation from Arthrobacter sp. strain IF1 were optimized and synthesized and then reconstructed into Escherichia coli by a multi-monocistronic vector to obtain recombinant BL-fpd that could degrade 4-FP efficiently. Under optimized induction conditions (inducing the strain by 2 g/L L-arabinose and 1 mM IPTG at 37 degrees C), BL-fpd could completely degrade 2 mM 4-FP, 4-chlorophenol, 4-bromophenol, and 4-nitrophenol into beta-ketoadipate, which could be further metabolized by the bacteria. FpdA2 showed the highest activity towards 4-bromophenol. The strain could completely degrade 1 mM 4-FP in industrial wastewater within 3 h. This study provided a promising strain for the degradation of 4-FP and some other 4-substituted phenols. The construction technologies of multi-monocistronic expression vector may also be used to construct other organic pollutants degrading bacteria.

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