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Genome-wide identification, immune response profile and functional characterization of IL-10 from spotted knifejaw (Oplegnathus punctatus) during host defense against bacterial and viral infection

文献类型： 外文期刊

作者： Wang, Jie 1 ; Chen, Zhangfan 1 ; Li, Ming 1 ; Zhang, Ziwei 1 ; Song, Yu 1 ; Xu, Wenteng 1 ; Wang, Lei 1 ; Chen, Songlin 1 ;

作者机构： 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Chinese Acad Fishery Sci CAFS, Yellow Sea Fisheries Res Inst, Qingdao 266237, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

3.Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Peoples R China

4.Shandong Key Lab Marine Fisheries Biotechnol & Ge, Qingdao 266071, Peoples R China

关键词： Interleukin-10; Oplegnathus punctatus; Vibrio harveyi; Spotted knifejaw iridovirus; Innate immune response

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.622； 五年影响因子：4.799 ）

ISSN： 1050-4648

年卷期： 2022 年 124 卷

页码：

收录情况： SCI

摘要： Interleukin 10 (IL-10), a pleiotropic cytokine, plays an essential role in multiple immunity responses. In the current study, the sequences of IL-10 family were identified from spotted knifejaw (Oplegnathus punctatus) whole genome, and O. punctatus IL-10 (OpIL-10) was cloned and characterized. OpIL-10 encodes 187 amino acids with a typical IL-10 family signature motif and predicted a-helices. It shared high identities with Notolabrus celidotus IL-10 and Epinephelus Lanceolatus IL-10. OpIL-10 was widely detected in healthy tissues, with the abundant expression in liver and skin. It was significantly up-regulated in the six immune-related tissues (liver, spleen, kidney, intestine, gill and skin) after infection against Vibrio harveyi and spotted knifejaw iridovirus (SKIV). Dual-luciferase analysis showed that OpIL-10 overexpression could suppress the activity of NF-kappa B. Meanwhile, OpIL-10 knockdown caused the down-regulation of five immune-related genes in JAK2/STAT3 signaling pathway and NF-kappa B signaling pathway, including IL-10R2, TYK2, STAT3, NOD2, and I kappa B. In addition, LPS and poly I:C stimulated expression of pro-inflammatory cytokines, including IL-6, IL-1 beta, IL-8, and IL-12, were lower with recombinant OpIL-10 (rOpIL-10) than the control group, indicating the anti-inflammatory roles of rOpIL-10. Taken together, these results indicated OpIL-10 as a negative regulator in the inflammatory responses of spotted knifejaw against bacterial and viral infection, which would help us better understand the role of IL-10 in teleost immunity.

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