Preparation of a novel sulfated glycopeptide complex and inhibiting L1210 cell lines property in vitro
文献类型: 外文期刊
作者: Ye, LiBin 1 ; Zhang, JingSong 1 ; Zhou, Shuai 1 ; Wang, Sheng 3 ; Wu, Di 4 ; Pan, YingJie 4 ;
作者机构: 1.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201106, Peoples R China
2.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China
3.Cent S Univ Forestry & Technol, Changsha 410004, Hunan, Peoples R China
4.Shanghai Fisheries Univ, Coll Food Sci, Shanghai 200090, Peoples R China
关键词: Ganoderma lucidum;Glycopeptide;Sulfated derivative;Composition;Anti-tumour
期刊名称:CARBOHYDRATE POLYMERS ( 影响因子:9.381; 五年影响因子:8.678 )
ISSN:
年卷期:
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收录情况: SCI
摘要: A native glycopeptide, LZ-D-4, was purified from the fruiting bodies of Ganoderma lucidum, and its derivative, LZ-D-9, was prepared using sulfated agent (CSA: Pyr = 1:6). HPLC experiment showed that both of them were homogeneity and had molecular weights of 1.56 x 10~4 and 1.30 x 10~4 Da, respectively. Some properties including amino acid and monosaccharide compositions were investigated for the two compounds, along with anti-tumour bioactivity in vitro. The results indicated that two monosaccharides (Xyl and Ara) were completely destroyed and most of amino acids in LZ-D-4 were broken during sulfated process. Anti-tumour test in vitro showed that both complexes have properties to inhibit growth of L1210 cell lines and have similar bioactivities, but might have different mechanism.
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