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Plasmonic Gold Nanoshell-Assisted Laser Desorption/Ionization Mass Spectrometry for Small-Biomolecule Analysis and Tissue Imaging

文献类型： 外文期刊

作者： Du, Mingyi 1 ; Chen, Dong 1 ; Chen, Yingying 1 ; Huang, Yudi 1 ; Ma, Lianlian 1 ; Xie, Qingrong 1 ; Xu, Yizhu 1 ; Zhu, Xinhai 3 ; Chen, Zilong 3 ; Yin, Zhibin 4 ; Xu, Hanhong 1 ; Wu, Xinzhou 1 ;

作者机构： 1.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Peoples R China

2.South China Agr Univ, Key Lab Nat Pesticide & Chem Biol, Minist Educ, Guangzhou 510642, Peoples R China

3.Sun Yat Sen Univ, Instrumental Anal & Res Ctr, Guangzhou 510275, Peoples R China

4.Guangdong Acad Agr Sci, Agro Biol Gene Res Ctr, Guangzhou 510640, Peoples R China

关键词： SiO2@Au; core-shell nanoparticles; laser desorption/ionization; mass spectrometry imaging; tissue imaging

期刊名称：ACS APPLIED NANO MATERIALS （ 影响因子：6.14； 五年影响因子：6.104 ）

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收录情况： SCI

摘要： Plasmonic nanoshells have been acknowledged as efficient nanomaterials for laser desorption/ionization mass spectrometry (LDI-MS) detection of a wide range of small molecules, whereas their applications in mass spectrometry imaging (MSI) are less developed. In this work, we constructed and optimized SiO2@Au nanoshells with tailor-made shell structures and compositions for high-sensitivity LDI-MS analysis and a wide range of MSI applications. Owing to the synergistic effects of plasmonic shells with nanoscale roughness and specific crevice space for the selective trapping of small molecules and cations, SiO2@Au core-shell nanoparticles exhibit superior performance for the detection of a vast diversity of small molecules, induding amino acids, oligosaccharides, dyestuff and drugs, peptides, nucleosides, and poly(ethylene glycols). Compared with organic matrices, this method affords a high reduction in matrix interference, higher analyte coverage, lower detection limits ranging from fmol to pmol, and good repeatability with relative standard deviation (RSD) below 5%. Due to the nanoscale size and homogeneous deposition of SiO2@Au nanoshells, the spatial distribution of various smallmolecule metabolites can be visualized in strawberry tissues at a pixel size of 100 mu m without imaging artifacts. More valuably, the universality of SiO2@Au-assisted LDI-MSI is further demonstrated for mapping the lipid distribution within the whole-body tissues of zebrafish (Danio rerio), honeybees (Apis cerana), and mouse brain tissues in a spatially resolved manner at pixel sizes of 55, 30, and 50 mu m, respectively. These results facilitate the expansion of the abilities of plasmonic core-shell nanoparticles in real-case MSI applications. Taken together, the results indicate that the SiO2@Au nanoshells are expected to be promising and efficient nanomaterials with superior DI efficiency and imaging capabilities, especially in the environmental science and life science fields.

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