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Verification of an efficient genetic sex identification method in Portunus trituberculatus: Applied to study sex ratios in full-sib families

文献类型： 外文期刊

作者： Zhang, Wen 1 ; Lv, Jianjian 2 ; Ti, Xingbin 2 ; Zhao, Xiao Yan 2 ; Jin, Ling 2 ; Wu, Jie 2 ; Gao, Baoquan 2 ; Liu, Ping 2 ;

作者机构： 1.Dalian Ocean Univ, Coll Marine Technol & Environm, Dalian 116023, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Peoples R China

3.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, 1 Wenhai Rd, Qingdao, Peoples R China

关键词： Portunus trituberculatus; Sex-linked marker; Sex identification; Sex ratio

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2021 年 543 卷

页码：

收录情况： SCI

摘要： Portunus trituberculatus is one of the main mariculture crabs in China and has obvious sexual dimorphism. The development of a genetic sex identification method is key to clarifying the mechanisms of sex determination. Based on an insertion-deletion (inDel) marker closely linked to the sex of P. trituberculatus, we successfully established a sex identification method with the advantages of high efficiency, low cost and low false-positive rates using conventional PCR and agarose gel electrophoresis. Based on the method, the sex ratio of seven full-sib families at different developmental stages were analyzed, including zoea, megalopa and II juvenile crab. Our results showed that the sex ratio of P. trituberculatus in early development stages approximated the theoretical sex ratio (1:1) of the XY sex determination system, although there were three families with sex ratio deviations from 1:1 at the juvenile stage. This study is important for detailing the mechanisms of sex determination, and is also conducive to the development of sex-control technologies in this species.

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