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Ultrasensitive detection porcine transmissible gastroenteritis virus based on the dual-photoelectrode photofuel cell with in-situ isothermal amplification

文献类型： 外文期刊

作者： Heng, Huadong 1 ; Yuan, Ruishuang 2 ; Ma, Hanyu 1 ; Li, Bin 3 ; Wang, Kun 1 ;

作者机构： 1.Jiangsu Univ, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China

2.Jiangsu Univ, Sch Agr Engn, Zhenjiang 212013, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Peoples R China

关键词： Electrochemical; Loop-mediated isothermal amplification; Dual-photoelectrode; Self-powered biosensors; Transmissible gastroenteritis virus

期刊名称：JOURNAL OF ELECTROANALYTICAL CHEMISTRY （ 影响因子：4.5； 五年影响因子：4.2 ）

ISSN： 1572-6657

年卷期： 2023 年 948 卷

页码：

收录情况： SCI

摘要： Loop-mediated isothermal amplification (LAMP) as a nucleic acid detection technology has many advantages than conventional polymerase chain reaction in terms of sensitivity, specificity, and equipment requirements. And combining photocatalytic fuel cell (PFC) power density readout with high sensitivity, we developed a LAMP-based dual-photoelectrode photocatalytic fuel cell based self-powered biosensors (LAMP PFC-SPBs) for the ultra-sensitive detection of transmissible gastroenteritis virus (TGEV). In this sensor, the specially designed primers were immobilized on the photosensitive material CdS-modified sensing substrate with a distinct electrochemical signal and amplified TGEV within 1 h. A wide detection range of the LAMP PFC-SPBs was demonstrated, ranging from 0.075 fg mu L-1 to 7.5 ng mu L-1, with a lower detection limit of 0.025 fg mu L-1. In-situ LAMP PFC-SPBS had great merits in simplicity (no complex thermal cycles, no need for expensive instruments), sensitivity, specificity, and provides a new perspective on the combination of nucleic acid amplification and electrochemistry.

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