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Ultra-sensitive photoelectrochemical biosensor for determination of African swine fever virus based on surface plasmon resonance

文献类型： 外文期刊

作者： Yuan, Ruishuang 1 ; Wei, Jie 2 ; Geng, Rui 3 ; Li, Bin 4 ; Xiong, Wei 5 ; Fang, Xueen 6 ; Lu, Peng 3 ; Wang, Kun 1 ;

作者机构： 1.Jiangsu Univ, Sch Agr Engn, Key Lab Modern Agr Equipment & Technol, Minist Educ, Zhenjiang 212013, Peoples R China

2.Jiangsu Univ, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China

3.Jiangsu Univ, Sch Life Sci, Zhenjiang 212003, Peoples R China

4.Inst Vet Med, Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China

5.Tech Ctr Anim, Plant & Food Inspect & Quarantine Shanghai Customs, 1208 Minsheng Rd, Shanghai 200135, Peoples R China

6.Fudan Univ, Inst Biomed Sci, Dept Chem, Shanghai 200433, Peoples R China

关键词： African swine fever virus; Loop-mediated isothermal amplification; Bismuth; bismuth subcarbonate; Screen printing carbon electrode; Surface plasmonic resonance

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.2； 五年影响因子：5.9 ）

ISSN： 0003-2670

年卷期： 2023 年 1276 卷

页码：

收录情况： SCI

摘要： Sensitive and specific detection of African swine fever virus (ASFV) is crucial for agricultural production and economic development due to the mortality and infectivity. In this study, a bismuth induced enhanced photo electrochemical (PEC) biosensor based on in-situ loop mediated isothermal amplification (LAMP) was constructed using deposited bismuth nanoparticles loaded bismuth oxycarbonate (Bi/(BiO)2CO3) as photoactive material, using primers designed according to LAMP as recognition elements, and using in-situ LAMP to achieve nucleic acid amplification of target genes. As the Bi induced surface plasmon resonance (SPR) effect, enhanced light captures and effective electron hole separation, it could effectively enhance the photoelectric activity, so the prepared Bi/(BiO)2CO3 nanohybrid had higher photocurrent intensity and good stability. The constructed PEC biosensor has realized the detection of ASFV in real samples with good sensitivity, specificity and repeatability. In the range from 1.0 x 10-13 to 1.0 x 10-7 g/L, the photoelectric current decreased with the increase of the concentration of ASFV, and the detection limit was 3.0 x 10-14 g/L (about 0.048 copies/& mu;L). Combining the advantages of LAMP with the excellent performance of PEC, it provides a simple, economical and efficient method for nucleic acid diagnosis, and also provides a new idea for biosensor detection.

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