Characterization of Litopenaeus vannamei secreted protein acidic and rich in cysteine -like in WSSV infection
文献类型： 外文期刊
作者： Zou, Rui-Feng 1 ; Ren, Xing-Chao 1 ; Liu, Qing-Hui 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Qingdao Key Lab Mariculture Epidemiol & Biosecur, Yellow Sea Fisheries Res Inst, Key Lab Maricultural Organism Dis Control,Minist, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China
3.Shanghai Ocean Univ, Shanghai 201306, Peoples R China
关键词： Shrimp; SPARC-L; Sequence; WSSV; Apoptosis
期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）
ISSN： 0022-2011
年卷期： 2021 年 183 卷
页码：
收录情况： SCI
摘要： Secreted protein acidic and rich in cysteine (SPARC) is an extracellular and non-structural glycoprotein. In shrimp, a significant function of SPARC in WSSV infection remains unclear. In this study, the full-length cDNA sequence of a secreted protein acidic and rich in cysteine -like was cloned from shrimp Litopenaeus vannamei (named as LvSPARC-L). LvSPARC-L contained an open reading frame of 1002 bp, encoding 333 amino acids. Bioinformatics analysis showed that LvSPARC-L contained a SPARC Ca2+-binding region in the C-terminus, a Kazal-type serine protease inhibitor domain and a BUD22 domain. Tissue distribution assay indicated that LvSPARC-L generally expressed in all tissues selected with a higher expression in hemocyte, stomach and pleoplod. In hepatopancreas and intestine, the relative expression of LvSPARC-L was significantly up-regulated following the WSSV challenge. Besides, the relative expression of viral immediately early gene IE1 and a late gene VP28 was significantly increased in the LvSPARCL-silenced shrimp. Furthermore, the relative expression of LvP53 and LvCaspase3 was extremely decreased in the stomach of dsLvSPARC-L treated shrimp, while that of LvP38 was not affected significantly. All data together suggest that LvSPARC-L might play an antiviral role by regulating apoptosis.
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