文献类型： 外文期刊

作者： Tan, Lulin ¹ ; Chen, Zhaojun ⁴ ; Ruan, Yong ² ; Xu, Houqiang ¹ ;

作者机构： 1.Guizhou Univ, Coll Life Sci, Guiyang 550025, Peoples R China

2.Guizhou Univ, Key Lab Anim Genet Breeding & Reprod Plateau Mt R, Minist Educ, Guiyang, Peoples R China

3.Guizhou Acad Agr Sci, Guizhou Anim Husb & Vet Res Inst, Guiyang, Peoples R China

4.Guizhou Acad Agr Sci, Potato Inst Guizhou Prov, Guiyang, Peoples R China

关键词： Pork quality; miRNA; intramuscular preadipocyte differentiation; RNA-seq; miR-148a-3p

期刊名称：EPIGENETICS （ 影响因子：4.861； 五年影响因子：5.293 ）

ISSN： 1559-2294

年卷期：

页码：

收录情况： SCI

摘要： Intramuscular fat development is regulated by a series of complicated processes, with non-coding RNA (ncRNA) such as microRNA (miRNA) having a critical role during intramuscular preadipocyte proliferation and differentiation in pigs. In the present study, the miRNA expression profiles of intramuscular preadipocytes from the longissimus dorsi muscle of Chinese Guizhou Congjiang Xiang pigs were detected by RNA-seq during various differentiation stages, namely, day 0 (D0), day 4 (D4), and day 8 (D8). A total of 67, 95, and 16 differentially expressed (DE) miRNAs were detected between D4 and D0, D8 and D0, and D8 and D4, respectively. According to gene ontology and Kyoto Encyclopedia of Genes analysis, target genes of DE miRNAs were enriched in categories and pathways related to lipid metabolic process, lipid biosynthetic process, as well as the PI3K-Akt, AMPK, and MAPK signalling pathways. Notably, miR-148a-3p was differentially expressed, with highest expressed abundance in D0, D4, and D8. Overexpression of miR-148a-3p in intramuscular preadipocytes increased cell proliferation and differentiation, and decreased apoptosis, in comparison to the knockdown of miR-148a-3p in intramuscular preadipocytes. Luciferase activity assays, quantitative polymerase-chain reaction, and western blot analysis confirmed that miR-148a-3p regulated adipogenesis by repressing PPARGC1A expression. Accordingly, the effect of miR-148a-3p mimic was attenuated by overexpression of PPARGC1A intramuscular preadipocytes. Furthermore, miR-148a-3p promoted intramuscular preadipocyte differentiation by inhibiting the AMPK/ACC/CPT1C signalling pathway. Taken together, we identified expression profiles of miRNAs in intramuscular preadipocytes and determined that miR-148a-3p acted as a promoter of adipogenesis.