Alleviating Effects of Black Soybean Peptide on Oxidative Stress Injury Induced by Lead in PC12 Cells via Keap1/Nrf2/TXNIP Signaling Pathway
文献类型： 外文期刊
作者： Li, Ning 1 ; Wen, Liuding 1 ; Li, Tiange 1 ; Yang, Huijie 1 ; Qiao, Mingwu 1 ; Wang, Tianlin 1 ; Song, Lianjun 1 ; Huang, Xianqing 1 ; Li, Mingming 1 ; Bukyei, Erkigul 2 ; Wang, Fangyu 3 ;
作者机构： 1.Henan Agr Univ, Coll Food Sci & Technol, 63 Agr Rd, Zhengzhou 450000, Peoples R China
2.Mongolian State Univ Life Sci, Sch Engn & Technol, Dept Food Engn & Hydromech, Zaisan 53, Ulaanbaatar 17024, Mongolia
3.Henan Acad Agr Sci, Key Lab Anim Immunol, 116 Huayuan Rd, Zhengzhou 450002, Peoples R China
关键词： black soybean peptide; Pb; PC12 cells; Keap1; Nrf2; TXNIP; oxidative stress
期刊名称：NUTRIENTS （ 影响因子：6.706； 五年影响因子：7.185 ）
ISSN：
年卷期： 2022 年 14 卷 15 期
页码：
收录情况： SCI
摘要： Many researchers have found that Pb exposure can cause oxidative stress damage to the body's tissue. Black soybean peptide (BSP) has a variety of physiological functions, especially in terms of oxidative stress. Nevertheless, the mitigation function of BSPs on Pb-induced oxidative stress damage in PC12 cells has not been clearly defined. In this study, cell viability was detected by CCK8. Oxidative stress indicators, such as ROS, GSH/GSSG, MDA, SOD, CAT, GPx, and GR, were tested with biochemical kit. Protein expression of Keap1, Nrf2, and TXNIP was measured by Western blot. Compared with the control group, Pb reduced the cell viability of PC12 cells. However, BSP treatment significantly increased the viability of PC12 cells induced by lead exposure (p < 0.05). Lead can enrich the contents of MDA and ROS, but decrease the amount of CAT, SOD, GR, GPx, and GSH/GSSG in PC12 cells, while BSP can alleviate it (p < 0.05). Lead can enhance the expression of Keap1 and TXNIP proteins, but reduce Nrf2 expression. In contrast, BSPs reversed this phenomenon (p < 0.05). BSPs can alleviate oxidative stress injury induced by lead in PC12 cells through the Keap1/Nrf2/TXNIP signaling pathway.
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