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A monoclonal antibody neutralizes pesudorabies virus by blocking gD binding to the receptor nectin-1

文献类型： 外文期刊

作者： Zhang, Teng 1 ; Liu, Yunchao 1 ; Chen, Yumei 2 ; Wang, Jucai 1 ; Feng, Hua 1 ; Wei, Qiang 1 ; Zhao, Shuangshuang 1 ; Yang 1 ;

作者机构： 1.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou, Peoples R China

2.Zhengzhou Univ, Sch Life Sci, Zhengzhou, Peoples R China

3.Northwest A&F Univ, Coll Vet Med, Yangling, Shaanxi, Peoples R China

4.Henan Zhongze Biol Engn Co Ltd, Zhengzhou, Peoples R China

5.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou, Peoples R China

6.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

关键词： Pseudorabies virus; Neutralizing mAbs; Therapeutic; Nectin-1

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2021 年 188 卷

页码：

收录情况： SCI

摘要： Pseudorabies virus (PRV) was isolated from some human cases recently and the infected patients manifested respiratory dysfunction and acute neurological symptoms. However, no effective drug or vaccine, preventing the progression of PRV infection, is available. Nectin-1 was the only reported receptor for PRV cell entry both swine and human origin, representing an excellent target to block PRV infection, and especially its transmission from pigs to humans. A PRV-gD specific mAbs (10B6) was isolated from hybridomas and its neutralizing activities in vitro and in vivo were determined. 10B6 exhibited effective neutralizing activities in vitro with IC50 = 2.514 mu g/ ml and 4.297 mu g/ml in the presence and absence of complement. And in vivo, 10B6 provided 100% protection against PRV lethal challenge with a dose of 15 mg/kg. Further, 10B6 could bind to a conserved epitope, (316)QPAEPFP(322), locating in gD pro-fusion domain, and finally blocks the binding of PRV-gD to nectin-1. Moreover, 10B6 showed an effective inhibition on PRV cell-attachment in a cell type-independent manner and could also block the virus spreading among cells. 10B6 exhibited effectively neutralizing activities to Chinese PRV variant strain in vitro and in vivo by blocking gD binding to nectin-1, implied both prophylactic and therapeutic interventions against PRV infections.

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