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Identification of genotype 3 hepatitis E virus in fecal samples from a pig farm located in a Shanghai suburb

文献类型： 外文期刊

作者： Ning, Haiqiang 1 ; Niu, Zhongxiang 2 ; Yu, Ruisong 2 ; Zhang, Peihua 2 ; Dong, Shijuan 2 ; Li, Zhen 3 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Sci & Vet Med, Shanghai 201106, Peoples R China

2.Shanghai Acad Agr Sci, Inst Anim Sci & Vet Med, Shanghai 201106, Peoples R China; Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; Shandong Agr Univ, Coll Anim Sci, Shandong 271018, Peoples R China

3.Shanghai Acad Agr Sci, Inst Anim Sci & Vet Med, Sh

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

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收录情况： SCI

摘要： Strains of hepatitis E virus (HEV) genotypes 1 and 4 have been detected on the Chinese mainland although there have been no previous reports of zoonotic genotype 3 HEV. In the present study, 65 swine fecal specimens were collected from five pig farms located in different Shanghai suburbs. RT-PCR and nested PCR were undertaken using partial nucleotide sequences of Open Reading Frame 2 (ORF2) of HEV to detect HEV RNA. Genetic analysis was based on alignments of an amplified 150-nt ORF2 sequence. RT-PCR revealed 15 HEV positive samples among 65-pig fecal specimens examined. Phylogenetic analysis of the amplified sequences indicated seven HEV strains belonged to genotype 3 and eight strains to genotype 4. This is the first time that genotype 3 hepatitis E virus has been identified on the Chinese mainland.

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