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A first set of gene-derived polymorphic microsatellite markers in half-smooth tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

作者： Ma, Hongyu 1 ; Chen, Songlin 1 ; Ma, Lingbo 2 ; Hu, Qiaomu 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China

2.Chinese Acad Fishery Sci, E China Sea Fisheries Res Inst, Minist Agr, Key Lab Marine & Estuarine Fisheries Resources &, Shanghai 200090, Peoples R China

关键词： gene: polymorphism;allele;polymorphic microsatellite marker: polymorphism;Hardy-Weinberg equilibrium;GenBank database;Bonferroni correction

期刊名称：ENVIRONMENTAL BIOLOGY OF FISHES （ 影响因子：1.844； 五年影响因子：1.839 ）

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收录情况： SCI

摘要： Half-smooth tongue sole (Cynoglossus semilaevis) is a commercially important marine fish species in China. A set of type I microsatellite markers were identified through bioinformatic mining of the GenBank database. Thirteen of these markers showed polymorphisms through genotyping a sample of 30 individuals. A total of 47 alleles were detected, with an average of 3.6 alleles per locus. The number of alleles, observed and expected heterozygosity per locus ranged from two to five, from 0.14 to 0.93 and from 0.27 to 0.77, respectively. Three loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction (P < 0.0038) and no significant linkage disequilibrum between pairs of loci was found. The markers identified in this study will contribute to construction of genetic linkage maps and mapping of quantitative trait loci (QTL) of C. semilaevis.

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