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Secretory expression and characterization of a soluble laccase from the Ganoderma lucidum strain 7071-9 in Pichia pastoris

文献类型: 外文期刊

作者: Sun, Jing 1 ; Peng, Ri-He 2 ; Xiong, Ai-Sheng 2 ; Tian, Yongsheng 2 ; Zhao, Wei 3 ; Xu, Hu 1 ; Liu, Da-Tong 1 ; Chen, Jia 1 ;

作者机构: 1.Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R China

2.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key Lab Appl Mycol Resources & Utilizat, Shanghai Key Lab Agr Genet & Breeding,Minist Agr, Shanghai 201106, Peoples R China

3.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key

关键词: Laccase;Ganoderma lucidum;Secretory expression;Protein purification;Decolorization

期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Laccases are strong oxidizing enzymes that oxidize chlorinated phenols, synthetic dyes, pesticides, polycyclic aromatic hydrocarbons as well as a very wide range of other compounds with high redox potential. Based on the bias of genetic codons between fungus and yeast, we synthesized a laccase gene GlLCCI, originated from Ganoderma lucidum using optimized codons and a PCR-based two-step DNA synthesis method. The recombinant laccase, GlLCCI was successfully over-expressed in yeast, Pichia pastoris, with an alcohol oxidase1 promoter. The recombinant GlLCCI has a molecular mass of approximately 58 kDa. The K (m) values of GlLCCI for 2-2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and guaiacol were 0.9665, and 1.1122 mM, respectively. The V (max) of GlLCCI for both substrates was 3,024 and 82.13 mu M mg(-1) min(-1). When ABTS was used as a substrate, the enzyme had an optimal temperature of approximately 55A degrees C. The enzyme was detected over pH values from 2 to 8. The enzyme was strongly activated by K+, Na+, Cu2+ and mannitol. Six amino acids (alanine, histidine, glycine, arginine, aspartate and phenylalanine) increased the catalytic ability of the enzyme. The activity of laccase was obviously inhibited by Fe2+, Fe3+, sodium hydrosulphite, and sodium azide. Additionally, under optimal conditions, GlLCCI decolorized 37.62 mg l(-1) of azo dye methyl orange (MO) in cultural medium. With a high MO degradation ability, GlLCCI may have potential in the treatment of industrial effluent containing azo dye MO.

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