您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Secretory expression and characterization of a soluble laccase from the Ganoderma lucidum strain 7071-9 in Pichia pastoris

文献类型： 外文期刊

作者： Sun, Jing 1 ; Peng, Ri-He 2 ; Xiong, Ai-Sheng 2 ; Tian, Yongsheng 2 ; Zhao, Wei 3 ; Xu, Hu 1 ; Liu, Da-Tong 1 ; Chen, Jia 1 ;

作者机构： 1.Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R China

2.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key Lab Appl Mycol Resources & Utilizat, Shanghai Key Lab Agr Genet & Breeding,Minist Agr, Shanghai 201106, Peoples R China

3.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key

关键词： Laccase;Ganoderma lucidum;Secretory expression;Protein purification;Decolorization

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Laccases are strong oxidizing enzymes that oxidize chlorinated phenols, synthetic dyes, pesticides, polycyclic aromatic hydrocarbons as well as a very wide range of other compounds with high redox potential. Based on the bias of genetic codons between fungus and yeast, we synthesized a laccase gene GlLCCI, originated from Ganoderma lucidum using optimized codons and a PCR-based two-step DNA synthesis method. The recombinant laccase, GlLCCI was successfully over-expressed in yeast, Pichia pastoris, with an alcohol oxidase1 promoter. The recombinant GlLCCI has a molecular mass of approximately 58 kDa. The K (m) values of GlLCCI for 2-2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and guaiacol were 0.9665, and 1.1122 mM, respectively. The V (max) of GlLCCI for both substrates was 3,024 and 82.13 mu M mg(-1) min(-1). When ABTS was used as a substrate, the enzyme had an optimal temperature of approximately 55A degrees C. The enzyme was detected over pH values from 2 to 8. The enzyme was strongly activated by K+, Na+, Cu2+ and mannitol. Six amino acids (alanine, histidine, glycine, arginine, aspartate and phenylalanine) increased the catalytic ability of the enzyme. The activity of laccase was obviously inhibited by Fe2+, Fe3+, sodium hydrosulphite, and sodium azide. Additionally, under optimal conditions, GlLCCI decolorized 37.62 mg l(-1) of azo dye methyl orange (MO) in cultural medium. With a high MO degradation ability, GlLCCI may have potential in the treatment of industrial effluent containing azo dye MO.

  • 相关文献

[1]Production of IgM by B Cells After Stimulation with GLIS from Ganoderma lucidum Mushroom. QINGJIU TANG,JINGSONG ZHANG,WERNER REUTTER,HUA FAN,Shanghai Academy of Agricultural Sciences(SAAS); Shanghai Academy of Agricultural Sciences(SAAS);. 2005

[2]XYLEM FORMATION AND LIGNIFICATION IN TREES AND MODEL SPECIES. Deborah Goffner,Taku Demura,Hiroo Fukuda,Alain M. Boudet,Alain Jauneau,Catherine Digonnet,Philippe Ranocha,Christophe Pineau,Magalie Pichon,Edouard Pesquet. 2001

[3]Induction of apoptosis in the SW620 colon carcinoma cell line by triterpene-enriched extracts from Ganoderma lucidum through activation of caspase-3. Ji, Zhe,Tang, Qingju,Hao, Ruixa,Zhang, Jinsong,Ji, Zhe,Pan, Yingje. 2011

[4]Purification, NMR Study and Immunostimulating Property of a Fucogalactan from the Fruiting Bodies of Ganoderma lucidum. Ye, Libin,Zhang, JingSong,Yang, Yan,Zhou, Shuai,Jia, Wei,Hao, RuiXia,Ye, Libin,Zhou, Kan,Pan, Yinglie. 2008

[5]Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers. Chao Wen-zheng,Yu Ling,Tang Chuan-hong,Zhang Jing-song,Tang Chuan-hong,Honda, Yoichi. 2018

[6]Isolation, Purification, and Immunological Activities of a Low-Molecular-Weight Polysaccharide from the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Higher Basidiomycetes). Zhu, Lina,Luo, Xi,Tang, Qingjiu,Liu, Yanfang,Zhou, Shuai,Yang, Yan,Zhang, Jingsong,Zhu, Lina,Luo, Xi,Tang, Qingjiu,Liu, Yanfang,Zhou, Shuai,Yang, Yan,Zhang, Jingsong,Zhu, Lina,Luo, Xi. 2013

[7]A novel Ganoderma lucidum G0119 fermentation strategy for enhanced triterpenes production by statistical process optimization and addition of oleic acid. Feng, Jie,Zhang, Jing-Song,Feng, Na,Yan, Meng-Qiu,Yang, Yan,Jia, Wei,Lin, Chi-Chung,Feng, Jie,Zhang, Jing-Song,Feng, Na,Yan, Meng-Qiu,Yang, Yan,Jia, Wei,Lin, Chi-Chung,Feng, Jie,Zhang, Jing-Song,Feng, Na,Yan, Meng-Qiu,Yang, Yan,Jia, Wei,Lin, Chi-Chung.

[8]Molecular cloning, characterization, and function analysis of a mevalonate pyrophosphate decarboxylase gene from Ganoderma lucidum. Shi, Liang,Qin, Lei,Ren, Ang,Fang, Xing,Mu, Dashuai,Zhao, Mingwen,Xu, Yingjie,Tan, Qi.

[9]Structural elucidation of the polysaccharide moiety of a glycopeptide (GLPCW-II) from Ganoderma lucidum fruiting bodies. Ye, LiBin,Zhang, JingSong,Tang, QingJiu,Liu, YanFang,Ye, LiBin,Du, XiuJui,Ye, XiJun,Gong, ChunYu,Pan, YingJie.

[10]Structural characterization and immuno-enhancing activity of a highly branched water-soluble beta-glucan from the spores of Ganoderma lucidum. Wang, Yatao,Liu, Yanfang,Zhou, Shuai,Zhang, Zhong,Wu, Di,Yan, Mengqiu,Tang, Qingjiu,Zhang, Jingsong,Wang, Yatao,Yan, Mengqiu,Yu, Huazheng.

[11]An Unstructured Kinetic Model for the Improvement of Triterpenes Production by Ganoderma lucidum G0119 Based on Nitrogen Source Effect. Feng, Jie,Zhang, Jing-Song,Jia, Wei,Yang, Yan,Liu, Fang,Lin, Chi-Chung.

[12]Preparation of a novel sulfated glycopeptide complex and inhibiting L1210 cell lines property in vitro. Ye, LiBin,Zhang, JingSong,Zhou, Shuai,Ye, LiBin,Wang, Sheng,Wu, Di,Pan, YingJie.

[13]Cloning and characterization of squalene synthase (SQS) gene from Ganoderma lucidum. Zhao, Ming-Wen,Liang, Wan-Qi,Zhang, Da-Bing,Wang, Nan,Wang, Chen-Guang,Pan, Ying-Jie. 2007

[14]NMR characterization for polysaccharide moiety of a glycopeptide. Zhang, Jingsong,Ye, Libin,Li, Jianrong,Pan, Yingjie. 2010

[15]Development of a simple and efficient transformation system for the basidiomycetous medicinal fungus Ganoderma lucidum. Shi, Liang,Fang, Xing,Li, Mengjiao,Mu, Dashuai,Ren, Ang,Zhao, Mingwen,Tan, Qi. 2012

[16]Immunomodulation of Bone Marrow Macrophages by GLIS, a Proteoglycan Fraction from Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidium (W.Curt.: Fr.) P. Karst.. Ji, Zhe,Tang, Qingjiu,Zhang, Jinsong,Yang, Yan,Liu, Yanfang,Ji, Zhe,Pan, Yingjie. 2011

[17]Physicochemical characterization of a high molecular weight bioactive beta-D-glucan from the fruiting bodies of Ganoderma lucidum. Liu, Yanfang,Zhang, Jingsong,Tang, Qingjiu,Yang, Yan,Wu, Di,Guo, Qingbin,Wang, Qi,Cui, Steve W.. 2014

[18]Biochemical characterization of a proteoglycan complex from an edible mushroom Ganoderma lucidum fruiting bodies and its immunoregulatory activity. Ye, LiBin,Zheng, Xiaolin,Wang, Xiangyang,Li, Jianrong,Zhang, Jingsong,Tang, Qingqiu,Yang, Yan,Liu, YanFang,Pan, YingJie.

[19]A New Temperature Control Shifting Strategy for Enhanced Triterpene Production by Ganoderma lucidum G0119 Based on Submerged Liquid Fermentation. Feng, Jie,Feng, Na,Zhang, Jing-Song,Yang, Yan,Jia, Wei,Lin, Chi-Chung,Feng, Jie,Feng, Na,Zhang, Jing-Song,Yang, Yan,Jia, Wei,Lin, Chi-Chung,Feng, Jie,Feng, Na,Zhang, Jing-Song,Yang, Yan,Jia, Wei,Lin, Chi-Chung.

[20]Structural Characterisation Of A Heteropolysaccharide By Nmr Spectra. Ye, LiBin,Zhang, JingSong,Yang, Yan,Zhou, Shuai,Liu, YanFang,Tang, Qinglui,Du, Xiuju,Chen, Hui,Ye, LiBin,Du, Xiuju,Pan, YingJie.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台