Expression and characterization of a recombinant 2,3-dihydroxybiphenyl-1,2-dioxygenase from Pseudomonas
文献类型： 外文期刊
作者： Xiong, Fei 1 ; Shuai, Jian-Jun 1 ; Jin, Xiao-Fen 3 ; Zhang, Jian 4 ; Sun, Jing 2 ; Peng, Ri-He 3 ; Yao, Quan-Hong 3 ; Xio 1 ;
作者机构： 1.Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R China
2.Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
3.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China
4.Alberta Innovates Technol Futures, Vegreville, AB T9C 1T4, Canada
关键词： PsbphCI gene;2;3-dihydroxybiphenyl-1;2-dioxygenase;Synthesized gene;2;3-dihydroxybiphenyl;HPLC
期刊名称：MOLECULAR & CELLULAR TOXICOLOGY （ 影响因子：1.08； 五年影响因子：1.184 ）
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收录情况： SCI
摘要： The 2, 3-dihydroxybiphenyl-1,2-dioxygenase, which can degrade the 2,3-dihydroxybiphenyl, was encoded by a synthesized PsbphCI gene. The PsbphCI gene was transformed into Escherichia coli and the encoding protein purified, had a molecular mass of similar to 32 kDa as determined by SDS-PAGE. The optimum pH for the purified enzyme at 20A degrees C was 9.0, and the optimal temperature at pH 8.0 was 30A degrees C. Subsequently, the PsbphCI gene was transformed into Pseudomonas putida sp. to verify the degradation of 2,3-dihydroxybiphenyl by HPLC. The transgenic EG11 strain degraded 65.20% of the 2,3-DHBP after 2 minutes at 30A degrees C, while the wild-type EG11 strain degraded only 37.75%. This study provides guidance for the cultivation of bioengineered biphenyl/PCBs-degrading bacteria which can be applied to the biodegradation of environmental biphenyl/PCBs contamination.
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