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Heavy Metal Influence on BDE-47 Uptake in the Human KERTr Keratinocyte Cell Line

文献类型: 外文期刊

作者: Kang, Yuan 1 ; Yin, Yi 4 ; Zhang, Qiu Yun 1 ; Li, Lai Sheng 1 ; Zeng, Li Xuan 1 ; Luo, Ji Wen 1 ; Wong, Ming Hung 2 ;

作者机构: 1.S China Normal Univ, Sch Chem & Environm, Key Lab Theoret Chem Environm, Minist Educ,Higher Educ Mega Ctr, Guangzhou 510006, Guangdong, Peoples R China

2.Croucher Inst Environm Sci, Kowloon Tong, Hong Kong, Peoples R China

3.Dept Biol, Kowloon Tong, Hong Kong, Peoples R China

4.Chinese Acad

关键词: uptake;PBDEs;KERTr;heavy metals

期刊名称:ENVIRONMENTAL TOXICOLOGY ( 影响因子:4.119; 五年影响因子:3.404 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Significant correlations between concentrations of PBDEs and heavy metals were observed in the human body. However, there is a lack of evidence on the linkage between the uptake of heavy metals and PBDEs. This study is the first report on the BDE-47 uptake profile in a human cell line. Hg and As exposures to KERTr (human skin derived keratinocyte) did not significantly (p[0.05) affect the uptake of BDE47, whereas Pb and Cd significantly (p\0.05) affected the uptake of BDE-47 in KERTr. The change in Km was minor after exposure to all heavy metals. The maximum transport rate (V_(max)) after exposure to Pb (V_(max): 5.23 ± 0.49) and As (V_(max): 4.95 ± 0.60) was significantly increased when compared with the background of the KERTr cell line (V_(max): 4.07 ± 0.35). Real-time RT-PCR indicated that OATP-B, OATP-D, and OATP-E were expressed in the KERTr cell line. The upregulation or downregulation of OATP B and D genes were minor after exposure to heavy metals, but the OATP E gene was upregulated by three to fourfold in KERTr cell line after exposure to Pb an Cd, which may explain the significant increase of uptake of BDE-47 in KERTr after exposures to Pb and Cd. This study indicated that the uptake effects should be considered when performing risk assessment of human exposure to PBDEs and heavy metal. # 2013 Wiley Periodicals, Inc. Environ Toxicol 29: 354-361, 2014.

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