您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Enhanced porcine circovirus Cap protein production by Pichia pastoris with a fuzzy logic DO control based methanol/sorbitol co-feeding induction strategy

文献类型： 外文期刊

作者： Ding, Jian 2 ; Zhang, Chunling 1 ; Gao, Minjie 2 ; Hou, Guoli 2 ; Liang, Kexue 2 ; Li, Chunhua 1 ; Ni, Jianping 1 ; Li, Z 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Res, Shanghai 201106, Peoples R China

2.Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Peoples R China

关键词： Cap protein;DO;Fermentation;Fuzzy control;Metabolic analysis

期刊名称：JOURNAL OF BIOTECHNOLOGY （ 影响因子：3.307； 五年影响因子：3.778 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Porcine circovirus Cap protein production by P. pastoris with strong AOX promoter suffered with the problems with traditional pure methanol induction: (1) inefficient methanol metabolism; (2) extensive oxygen supply load; (3) difficulty in stable DO control; (4) low protein titer. In this study, based on the difference of DO change patterns in response to methanol and sorbitol additions, a novel fuzzy control system was proposed to automatically regulate the co-feeding rates of methanol and sorbitol for efficient Cap protein induction. With aid of the proposed control system when setting DO control level at 10%, overall fermentation performance was significantly improved: (1) DO could be stably controlled under mild aeration condition; (2) methanol consumption rate could be restricted at moderate level and the major enzymes involved with methanol metabolism were largely activated; (3) Cap protein concentration reached a highest level of 198 mg/L, which was about 64% increase over the best one using the pure methanol induction strategies. (C) 2014 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Enhanced porcine interferon-alpha production by recombinant Pichia pastoris with a combinational control strategy of low induction temperature and high dissolved oxygen concentration. Jin, Hu,Liu, Guoqiang,Duan, Zuoying,Shi, Zhongping,Ye, Xiaofei,Li, Zhen. 2010

[2]Establishment and Analysis of the Fermentation Model of Phellinus Igniarius. Ziping Zhu,Na Li,Jinbin Wang. 2012

[3]Improvement of Lactic Cell Production. S. DESMONS,H. KRHOUZ,P. EVRARD,P. THONART. 1998

[4]Establishment and Analysis of the Fermentation Model of Phellinus Igniarius. Ziping Zhu,Na Li,Jinbin Wang,Xueming Tang. 2012

[5]Hedges and woody strips browsing by cattle on pasture in Wallonia, Belgium. Sophie Vandermeulen,Mathilde Pascal,Christian Marche,Arnaud Pilet,Yves Beckers,Jerome Bindelle. 2013

[6]An Unstructured Kinetic Model for the Improvement of Triterpenes Production by Ganoderma lucidum G0119 Based on Nitrogen Source Effect. Feng, Jie,Zhang, Jing-Song,Jia, Wei,Yang, Yan,Liu, Fang,Lin, Chi-Chung.

[7]Improvement of ATP regeneration efficiency and operation stability in porcine interferon-alpha production by Pichia pastoris under lower induction temperature. Gao, Minjie,Wu, Jianrong,Zheng, Zhiyong,Shi, Zhongping,Zhan, Xiaobei,Dong, Shijuan,Yu, Ruisong. 2011

[8]Establishment and Analysis of the Fermentation Model of Phellinus Igniarius. Zhu, Ziping,Wang, Jinbin,Tang, Xueming,Li, Na. 2012

[9]Enhancing pIFN-alpha Production and Process Stability in Fed-Batch Culture of Pichia pastoris by Controlling the Methanol Concentration and Monitoring the Responses of OUR/DO Levels. Gao, Min-Jie,Zhan, Xiao-Bei,Zheng, Zhi-Yong,Wu, Jian-Rong,Shi, Zhong-Ping,Lin, Chi-Chung,Dong, Shi-Juan,Li, Zhen. 2013

[10]Isolation, Characterization, and Molecular Cloning of the cDNA Encoding a Novel Phytase from Aspergillus niger 113 and High Expression in Pichia pastoris. Xiong, AS,Yao, QH,Peng, RH,Li, M,Fan, HQ,Guo, MJ,Zhang, SL.

[11]Improving Performance and Operational Stability of Porcine Interferon-alpha Production by Pichia pastoris with Combinational Induction Strategy of Low Temperature and Methanol/Sorbitol Co-feeding. Gao, Min-Jie,Zhan, Xiao-Bei,Gao, Peng,Zhang, Xu,Shi, Zhong-Ping,Lin, Chi-Chung,Dong, Shi-Juan,Li, Zhen.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台