Expression and purification of half-smooth tongue sole (Cynoglossus semilaevis) CSDAZL protein
文献类型: 外文期刊
作者: Wang, Kailin 1 ; Zhang, Hong 1 ; Hu, Qiaomu 1 ; Shao, Changwei 1 ; Chen, Songlin 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
关键词: CSDAZL;Cynoglossus semilaevis;Fusion protein;Protein transfection
期刊名称:PROTEIN EXPRESSION AND PURIFICATION ( 影响因子:1.65; 五年影响因子:1.548 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: The csdazl gene is a sex related gene of half-smooth tongue sole (Cynoglossus semilaevis). Our research group have cloned full length cDNA of csdazl, and studied its expression pattern. To get the further information of csdazl, we constructed a prokaryotic expression plasmid, pET-32a-CSDAZL, expressed in Escherichia coli BL21 and purified the fusion protein by His Trap. In order to detect the biological activity of the fusion protein, we injected the protein with liposome into fish, and detected other sex-related genes' mRNA expression. The results showed that the expression levels of half-smooth tongue sole female-related genes Cyp19a and Foxl2 significantly decreased between 6 and 24 h; however, both genes' expressions returned to their normal levels 72 h after injection, indicating that recombinant CSDAZL protein could down-regulated the expression of female-related genes, Foxl2 and Cyp19a genes, implying that the fusion protein has biological activity and csdazl plays a role in sex differentiation by regulating sex related genes' expression. (C) 2014 Elsevier Inc. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
Differentially expressed proteins and microbial communities of the skin regulate disease resistance to Chinese tongue sole (Cynoglossus semilaevis)
作者:Wang, Lei;Tian, Min;Chen, Songlin;Wang, Lei;Tian, Min;Chen, Songlin;Wang, Lei;Tian, Min;Chen, Songlin;Tian, Min
关键词:Cynoglossus semilaevis; skin; proteome; microbial community; protein microbial interaction
-
Integrated transcriptomic and metabolomic analysis provides insights into the responses to Vibrio infection in Plectropomus leopardus
作者:Wang, Jie;Zhang, Junwei;Chen, Songlin;Zhou, Qian;Wang, Jie;Zhu, Chunhua;Wang, Shengpeng;Wang, Shengpeng
关键词:Plectropomus leopardus; Vibrio infection; Transcriptomics; Metabolomics; Bile acids; Eicosanoids
-
Establishment and characterization of cell line from fin of Chinense bahaba (Bahaba taipingensis) and transcriptome analysis of its response to megalocytivirus SKIV-SD strain infection
作者:Gong, Zhihong;Zhang, Qi;Wang, Na;Wang, Lei;Chen, Zhangfan;Chen, Songlin;Gong, Zhihong;Hu, Guobin;Zhang, Qi;Yan, Lin;Yan, Kuoqiu
关键词:Chinese bahaba; Fin; Cell line; BTF; Megalocytivirus
-
Effects of Inducible Nitric Oxide Synthase (iNOS) Gene Knockout on the Diversity, Composition, and Function of Gut Microbiota in Adult Zebrafish
作者:Huang, Yajuan;Chen, Yadong;Xie, Haisheng;Feng, Yidong;Bao, Baolong;Chen, Yadong;Chen, Songlin
关键词:inducible nitric oxide synthase; zebrafish; gut microbiota; 16S rRNA; immune
-
The Mitogenomic Landscape of Hexacorallia Corals: Insight into Their Slow Evolution
作者:Wei, Zhanfei;Shao, Changwei;Wei, Zhanfei;Yang, Yang;Zhang, Nannan;Liu, Shanshan;Meng, Liang;Yang, Yang;Meng, Lihui;Li, Yang
关键词:deep-sea corals; Hexacorallia; mitogenomes; phylogenetics
-
Molecular functional characterization of the setdb1 and its potential target gene sox5 illuminate the histone modification-mediated orchestration of gonadal development in Chinese tongue sole (Cynoglossus semilaevis)
作者:Yue, Bowen;Yue, Bowen;Wang, Hong-Yan;Huang, Yingyi;Li, Shuo;Ma, Wenxiu;Liu, Qian;Shao, Changwei;Wang, Hong-Yan;Huang, Yingyi;Li, Shuo;Ma, Wenxiu;Liu, Qian;Shao, Changwei;Shao, Changwei
关键词:Setdb1; Gonad; Sox5; Chinese tongue sole
-
Integrative omics reveals clues as to why Cynoglossus semilaevis pseudomales produce no W sperm
作者:Zhang, Yaqun;Wang, Liping;Xu, Feng;Ye, Zhan;Li, Hengde;Zhang, Yaqun;Wang, Liping;Xu, Feng;Ye, Zhan;Li, Hengde;Wang, Liping;Xu, Feng;Ye, Zhan;Chen, Songlin;Chen, Songlin;Chen, Songlin;Li, Hengde
关键词:Spermatogenesis; Transcriptome; Proteome; Ubiquitination; Pseudomale; Cynoglossus semilaevis