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Metabolic engineering of Arabidopsis for remediation of different polycyclic aromatic hydrocarbons using a hybrid bacterial dioxygenase complex

文献类型： 外文期刊

作者： Peng, Rihe 1 ; Fu, Xiaoyan 1 ; Tian, Yongsheng 1 ; Zhao, Wei 1 ; Zhu, Bo 1 ; Xu, Jing 1 ; Wang, Bo 1 ; Wang, Lijuan 2 ; Yao, 1 ;

作者机构： 1.Natl Ctr Plant Gene Res, Shanghai Acad Agr Sci, Agobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai, Peoples R China

2.Natl Ctr Plant Gene Res, Shanghai Acad Agr Sci, Agobiotech

关键词： Polycyclic aromatic hydrocarbons;Metabolic engineering;Hybrid naphthalene dioxygenase complex;Transgenic Arabidopsis;Phytoremediation

期刊名称：METABOLIC ENGINEERING （ 影响因子：9.783； 五年影响因子：9.566 ）

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收录情况： SCI

摘要： The widespread presence of polycyclic aromatic hydrocarbons (PAHs) and their potential harm to various organisms has generated interest in efficiently eliminating these compounds from the environment. Phytoremediation is an efficient technology for cleaning up pollutants. However, unlike microorganisms, plants lack the catabolic pathway for complete degradation of these dangerous groups of compounds. One way to enhance the potential of plants for remediation of these compounds is by transferring genes involved in xenobiotic degradation from microbes to plants. In this paper, four genes, namely nidA and nidB (encoding the large and small subunits of naphthalene dioxygenase of Mycobacterium vanbaalenii PYR-1) as well as NahAa and NahAb (encoding flavoprotein reductase and ferredoxin of the electrontransport chain of the Pseudomonas putida G7 naphthalene dioxygenase system), were transferred and ectopically expressed in Arabidopsis thaliana. Transgenic Arabidopsis plants overexpressing the heterozygous naphthalene dioxygenase system exhibited enhanced tolerance toward 2-4 rings PAHs. Transgenic plants assimilated PAHs from the culture media faster and accumulated less in vivo than wild-type plants. Furthermore, examination of metabolic intermediates by gas chromatography-mass spectrometry revealed that the naphthalene metabolic pathway in transgenic plants mainly involves the dioxygenase pathway. Taken together, our findings suggest that grafting the naphthalene dioxygenase complex into plants is a possible strategy to breed PAR-tolerant plants to efficiently degrade PAHs in the environment. (C) 2014 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

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[1]Phytoremediation of triphenylmethane dyes by overexpressing a Citrobacter sp triphenylmethane reductase in transgenic Arabidopsis. Fu, Xiao-Yan,Zhao, Wei,Xiong, Ai-Sheng,Tian, Yong-Sheng,Zhu, Bo,Peng, Ri-He,Yao, Quan-Hong. 2013

[2]Phytoremediation of 2,4,6-trinitrotoluene by Arabidopsis plants expressing a NAD(P)H-flavin nitroreductase from Enterobacter cloacae. You, Shuang-Hong,Zhu, Bo,Han, Hong-Juan,Wang, Bo,Peng, Ri-He,Yao, Quan-Hong.

[3]Enhancement of naphthalene tolerance in transgenic Arabidopsis plants overexpressing the ferredoxin-like protein (ADI1) from rice. Fu, Xiao-Yan,Zhu, Bo,Han, Hong-Juan,Zhao, Wei,Tian, Yong-Sheng,Peng, Ri-He,Yao, Quan-Hong.

[4]Microbial biodegradation of polyaromatic hydrocarbons. Peng, Ri-He,Xiong, Ai-Sheng,Xue, Yong,Fu, Xiao-Yan,Gao, Feng,Zhao, Wei,Tian, Yong-Sheng,Yao, Quan-Hong.

[5]Functional Characterization of aroA from Rhizobium leguminosarum with Significant Glyphosate Tolerance in Transgenic Arabidopsis. Han, Jing,Tian, Yong-Sheng,Xu, Jing,Wang, Li-Juan,Wang, Bo,Peng, Ri-He,Yao, Quan-Hong.

[6]Functional characterization of Class II 5-enopyruvylshikimate-3-phosphate synthase from Halothermothrix orenii H168 in Escherichia coli and transgenic Arabidopsis. Tian, Yong-Sheng,Xu, Jing,Xiong, Ai-Sheng,Zhao, Wei,Gao, Feng,Fu, Xiao-Yan,Peng, Ri-He,Yao, Quan-Hong.

[7]Novel Glyphosate-Resistant aroA Gene from Paracoccus denitrificans in Transgenic Arabidopsis. Tian, Y. -S.,Xiong, A. -S.,Tian, Y. -S.,Yao, Q. -H.,Peng, R. -H.,Xu, J.,Tian, Y. -S.,Xing, X. -J..

[8]Functional Characterization of 5-Enopyruvylshikimate-3-Phosphate Synthase from Alkaliphilus metalliredigens in Transgenic Arabidopsis. Xing, Xiao-Juan,Sun, Sheng,Xing, Xiao-Juan,Tian, Yong-Sheng,Peng, Ri-He,Xu, Jing,Zhao, Wei,Yao, Quan-Hong.

[9]Microarray analysis of the phytoremediation and phytosensing of occupational toxicant naphthalene. Peng, Ri-He,Xu, Ran-Ran,Fu, Xiao-Yan,Xiong, Ai-Sheng,Zhao, Wei,Tian, Yong-Sheng,Zhu, Bo,Jin, Xiao-Fen,Chen, Chen,Han, Hong-Juan,Yao, Quan-Hong.

[10]Phytoremediation potential of Arabidopsis with reference to acrylamide and microarray analysis of acrylamide-response genes. Gao, Jian-Jie,Peng, Ri-He,Zhu, Bo,Wang, Bo,Wang, Li-Juan,Xu, Jing,Sun, Miao,Yao, Quan-Hong.

[11]Enhancement of phenol stress tolerance in transgenic Arabidopsis plants overexpressing glutathione S-transferase. Xu, Jing,Tian, Yong-Sheng,Xing, Xiao-Juan,Xu, Zhi-Sheng,Zhu, Bo,Fu, Xiao-Yan,Peng, Ri-He,Yao, Quan-Hong.

[12]Improving Rice Ability to Degrade Different Polycyclic Aromatic Hydrocarbons Through Multigene Transformation of a Hybrid Dioxygenase System. Peng, Ri-He,Shi, Biao,Fu, Xiao-Yan,Tian, Yong-Sheng,Zhao, Wei,Zhu, Bo,Xu, Jing,Han, Hong-Juan,Yuan, Zhong-Yi,Liu, Kai-Hong,Yao, Quan-Hong.

[13]Identification of candidate genes involved in responses of Arabidopsis to polychlorinated biphenyls based on microarray analysis. Jin, Xiao-Fen,Shuai, Jian-Jun,Peng, Ri-He,Zhu, Bo,Fu, Xiao-Yan,Tian, Yong-Sheng,Zhao, Wei,Han, Hong-Juan,Chen, Chen,Xu, Jing,Yao, Quan-Hong,Xiong, Ai-Sheng,Qu, Shen-Chun.

[14]Analysis of gene expression profile of Arabidopsis genes under trichloroethylene stresses with the use of a full-length cDNA microarray. Zhu, Bo,Peng, Ri-He,Xiong, Ai-Sheng,Fu, Xiao-Yan,Zhao, Wei,Tian, Yong-Sheng,Jin, Xiao-Fen,Xue, Yong,Xu, Jing,Han, Hong-Juan,Chen, Chen,Gao, Jian-Jie,Yao, Quan-Hong.

[15]Phytoremediation and phytosensing of chemical contaminant, toluene: identification of the required target genes. Gao, Jian-Jie,Shen, Xue-Fang,Peng, Ri-He,Zhu, Bo,Xu, Jing,Han, Hong-juan,Yao, Quan-Hong,Shen, Xue-Fang.

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