Protective immune response against newly emerging goose tembusu virus infection induced by immunization with a recombinant envelope protein
文献类型： 外文期刊
作者： Zhao, D. 1 ; Huang, X. 1 ; Han, K. 1 ; Liu, Y. 1 ; Yang, J. 1 ; Liu, Q. 1 ; An, F. 1 ; Li, Y. 1 ;
作者机构： 1.Minist Agr, Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Nanjing, Jiangsu, Peoples R China
关键词： envelope protein;goose tembusu virus;immune response;neutralization;protection
期刊名称：LETTERS IN APPLIED MICROBIOLOGY （ 影响因子：2.858； 五年影响因子：2.776 ）
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收录情况： SCI
摘要： Flavivirus envelope protein locates at the outermost surface of viral particle and mediates virus entry and fusion infection, and plays an important role in eliciting neutralizing antibody. In this study, goose tembusu virus (GTV) E protein was expressed and purified from Escherichia coli. The immunological changes and protection efficiency of this protein serving as recombinant subunit vaccine were then tested. In ducks, recombinant E protein markedly elicited specific neutralizing antibody, stimulated the secretion of IL-2, TNF-alpha and IFN-gamma cytokines and promoted the proliferation of lymphocytes. Additionally, population of soluble CD4 and soluble CD8 molecules in sera significantly increased in response to this antigen. Moreover, virus challenge revealed that recombinant E protein contributed to protection against GTV challenge. Taken together, the data indicate that recombinant GTV E protein could induce significant humoral and cell-mediated responses and that recombinant E protein can serve as a potential subunit vaccine candidate against GTV.
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