Sperm of the giant grouper: cryopreservation, physiological and morphological analysis and application in hybridizations with red-spotted grouper
文献类型: 外文期刊
作者: Tian, Yongsheng 1 ; Jiang, Jing 1 ; Wang, Na 1 ; Qi, Wenshan 1 ; Zhai, Jieming; Li, Bo; Liang, You 1 ; Chen, Youmin 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R Chi
关键词: Cryopreservation;Crossbreeding;Epinephelus lanceolatus;Physiological characteristics;Sperm
期刊名称:JOURNAL OF REPRODUCTION AND DEVELOPMENT ( 影响因子:2.214; 五年影响因子:2.221 )
ISSN:
年卷期:
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收录情况: SCI
摘要: In order to develop excellent germplasm resources for giant grouper (Epinephelus lanceolatus), cryopreservation of giant grouper sperm was examined in the present study. Firstly, 13 kinds of sperm dilution (ELS1-3, EM1-2, TS-2, MPRS, ELRSO-6) were prepared with physiological salt, sucrose, glucose and fetal bovine serum. The physiological parameters of ELRS3 (ratio of fast motion, ratio of slow motion, time of fast motion, time of slow motion, lifespan and motility) and ELS3 (sperm ratio of slow motion, time of slow motion and motility) were significantly higher than those of the other dilutions (P < 0.05). Secondly, after adding 15% DMSO and 10% PBS to ELRS3 and ELS3, most physiological parameters of frozen sperm were also significantly higher than the other gradients (P < 0.05), and sperm motility was as high as 63.68 +/- 4.16% to 74.75 +/- 12.71% (fresh sperm motility, 80.70 +/- 1.37% to 80.71 +/- 1.49%). Mixed with the above dilutions, a final volume of 105 ml semen was cryopreserved. Finally, the sperm of giant grouper cryopreserved with cryoprotectants (ELRS3 + 15% DMSO + 10% FBS) was used for electron-microscopic observation and crossbreeding with red-spotted groupers (Epinephelus akaara). The electron-microscopic observation revealed that part of the frozen-thawed sperm was cryodamaged, e.g., flagellum fracturing and mitochondria falling out, while the ultrastructure of sperm membrane, mitochondria and flagellum remained intact. Also, the fertilization and hatchability rates of giant grouper frozen sperm and red-spotted grouper eggs were as high as 94.56% and 75.56%, respectively. Thus, a technique for cryopreservation of giant grouper sperm was successfully developed and applied to crossbreeding with red-spotted grouper eggs.
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