Phytoremediation of 2,4,6-trinitrotoluene by Arabidopsis plants expressing a NAD(P)H-flavin nitroreductase from Enterobacter cloacae
文献类型： 外文期刊
作者： You, Shuang-Hong 1 ; Zhu, Bo 2 ; Han, Hong-Juan 2 ; Wang, Bo 2 ; Peng, Ri-He 2 ; Yao, Quan-Hong 2 ;
作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China
2.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
关键词： Enterobacter cloacae;Phytoremediation;TNT;Transgenic Arabidopsis
期刊名称：PLANT BIOTECHNOLOGY REPORTS （ 影响因子：2.01； 五年影响因子：1.907 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： 2,4,6-Trinitrotoluene (TNT) is released into natural environment from demilitarization facilities, manufacturing, and explosive remnants of war; this compound is one of the most recalcitrant explosives. TNT contamination is associated with human health risks because TNT strongly causes mutagenicity and carcinogenicity. Unfortunately, effective and affordable technologies to remediate TNT-contaminated environments are insufficient. As such, studies have been conducted to develop strategies using plants to extract and detoxify TNT from environment. In this study, a system was designed to overcome high phytotoxicity of TNT by expressing a NAD(P) H-flavin nitroreductase from Enterobacter cloacae to investigate the possibility of TNT phytoremediation. The resulting transgenic Arabidopsis showed a remarkable improvement in the ability to tolerate, absorb, and detoxify TNT as evidenced by their growth condition. This study can be used as reference to facilitate the effective cleanup of TNT-contaminated sites.
- 相关文献
作者其他论文 更多>>
-
Synthesis of betanin by expression of the core betalain biosynthetic pathway in carrot
作者：Wang, Bo;Wang, Yahui;Deng, Yuanjie;Xiong, Aisheng;Wang, Bo;Yao, Quanhong
关键词：Daucus carota L.; Betanin; Biosynthesis; Metabolic engineering; Bioforti fication
-
The complete degradation of 1,2-dichloroethane in Escherichia coli by metabolic engineering
作者：Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong;Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong
关键词：Bioremediation; Engineered bacteria; Environmental pollution; Metabolic engineering; Synthetic biology
-
Ectopic expression of an Old Yellow Enzyme (OYE3) gene from Saccharomyces cerevisiae increases the tolerance and phytoremediation of 2-nitroaniline in rice
作者：Li, Zhenjun;Gao, Jianjie;Wang, Bo;Zhang, Hao;Tian, Yongsheng;Peng, Rihe;Yao, Quanhong
关键词：2-nitroaniline; Old Yellow Enzyme; Phytoremediation; Reactive oxygen species; Saccharomyces cerevisiae
-
Biosynthesis of melatonin from
l -tryptophan by an engineered microbial cell factory作者：Wang, Lijuan;Deng, Yongdong;Gao, Jianjie;Wang, Bo;Han, Hongjuan;Li, Zhenjun;Zhang, Wenhui;Wang, Yu;Fu, Xiaoyan;Peng, Rihe;Yao, Quanhong;Tian, Yongsheng;Xu, Jing;Wang, Lijuan;Deng, Yongdong;Peng, Rihe;Yao, Quanhong;Tian, Yongsheng;Xu, Jing
关键词：Biosynthesis; Melatonin;
l -Tryptophan (l -Trp); Escherichia coli; Metabolic engineering -
Metabolic engineering of Escherichia coli for 2,4-dinitrotoluene degradation
作者：Zhang, Wen-Hui;Deng, Yong-Dong;Zuo, Zhi-Hao;Tian, Yong-Sheng;Xu, Jing;Wang, Bo;Wang, Li-Juan;Han, Hong-Juan;Li, Zhen-Jun;Wang, Yu;Yao, Quan-Hong;Gao, Jian-Jie;Fu, Xiao-Yan;Peng, Ri-He;Zhang, Wen-Hui;Deng, Yong-Dong;Zuo, Zhi-Hao;Tian, Yong-Sheng;Xu, Jing;Wang, Bo;Wang, Li-Juan;Han, Hong-Juan;Li, Zhen-Jun;Wang, Yu;Yao, Quan-Hong;Gao, Jian-Jie;Fu, Xiao-Yan;Peng, Ri-He;Chen, Zhi-Feng
关键词：4-Dinitrotoluene; Re-synthesize gene; Complete degradation; Bioremediation; Escherichia coli
-
Creation of Environmentally Friendly Super "Dinitrotoluene Scavenger" Plants
作者：Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Zhu, Bo
关键词：2,4-dinitrotoluene (2,4-DNT); carbon neutrality; complete degradation; rapid phytoremediation; sustainable development goals
-
Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot
作者：Wang, Bo;Wang, Ya-Hui;Deng, Yuan-Jie;Xiong, Ai-Sheng;Wang, Bo;Yao, Quan-Hong
关键词：Genetically modified carrot; Betanin; Unintended effects; Photosynthesis; Transcriptome; Metabolomics
