Phytoremediation of 2,4,6-trinitrotoluene by Arabidopsis plants expressing a NAD(P)H-flavin nitroreductase from Enterobacter cloacae
文献类型: 外文期刊
作者: You, Shuang-Hong 1 ; Zhu, Bo 2 ; Han, Hong-Juan 2 ; Wang, Bo 2 ; Peng, Ri-He 2 ; Yao, Quan-Hong 2 ;
作者机构: 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China
2.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
关键词: Enterobacter cloacae;Phytoremediation;TNT;Transgenic Arabidopsis
期刊名称:PLANT BIOTECHNOLOGY REPORTS ( 影响因子:2.01; 五年影响因子:1.907 )
ISSN:
年卷期:
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收录情况: SCI
摘要: 2,4,6-Trinitrotoluene (TNT) is released into natural environment from demilitarization facilities, manufacturing, and explosive remnants of war; this compound is one of the most recalcitrant explosives. TNT contamination is associated with human health risks because TNT strongly causes mutagenicity and carcinogenicity. Unfortunately, effective and affordable technologies to remediate TNT-contaminated environments are insufficient. As such, studies have been conducted to develop strategies using plants to extract and detoxify TNT from environment. In this study, a system was designed to overcome high phytotoxicity of TNT by expressing a NAD(P) H-flavin nitroreductase from Enterobacter cloacae to investigate the possibility of TNT phytoremediation. The resulting transgenic Arabidopsis showed a remarkable improvement in the ability to tolerate, absorb, and detoxify TNT as evidenced by their growth condition. This study can be used as reference to facilitate the effective cleanup of TNT-contaminated sites.
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