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Aptamer-functionalized magnetic nanoparticles for simultaneous fluorometric determination of oxytetracycline and kanamycin

文献类型： 外文期刊

作者： Liu, Changbin 1 ; Lu, Chunxia 2 ; Tang, Zonggui 2 ; Chen, Xia 2 ; Wang, Guohong 2 ; Sun, Fengxia 2 ;

作者机构： 1.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Sci, Shihezi 832000, Peoples R China

2.Xinjiang Acad Agr & Reclamat Sci, Anal & Testing Ctr, Shihezi 832000, Peoples R China

关键词： Oxytetracycline;Kanamycin;Antibiotic;Aptamer;Magnetic nanoparticles (MNPs);Dual-color fluorescence;Food analysis

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）

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收录情况： SCI

摘要： This work describes a method for the simultaneous detection of oxytetracycline (OTC) and kanamycin (KMY) using aptamers acting as both recognition and separation elements, and complementary oligonucleotides labeled with a green emitting fluorophore (carboxyfluorescein, FAM) and a yellow emitting fluorophore (carboxy-X-rhodamine, ROX), respectively, as signal labels. An OTC aptamer and a KMY aptamer were immobilized on the surface of magnetic nanoparticles (MNPs) via avidin-biotin chemistry. The aptamers preferentially bind their respective targets and thereby cause the upconcentration of analytes. However, in their absence they bind fluorescently-tagged complementary oligonucleotide later added to the reaction system. This cause the NPs to become fluorescent, with emission peaks located at 520 and 608 nm, respectively. The effects of the concentration of avidin, aptamer, complementary oligonucleotide, incubation temperature and incubation time were optimized. Under the optimal conditions, linear relationships were obtained in the range of 1-50 nga (TM) mL(-1) for OTC and KMY, with limits of detection of 0.85 nga (TM) mL(-1) and 0.92 nga (TM) mL(-1), respectively. The method was applied to the analysis of pork, milk, and honey samples spiked with OTC and MKY. Recoveries ranged from 76.5 to 94.7 % and 77.8 to 93.1 %, respectively, and the relative standard deviation was < 10.0 %.

  • 相关文献

[1]Magnetic-nanobead-based competitive enzyme-linked aptamer assay for the analysis of oxytetracycline in food. Lu, Chunxia,Tang, Zonggui,Kang, Lichao,Sun, Fengxia,Lu, Chunxia,Tang, Zonggui,Kang, Lichao,Sun, Fengxia,Liu, Changbin.

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