您好,欢迎访问中国水产科学研究院 机构知识库!

Effects of cryopreservation on the survival rate of the seven-band grouper (Epinephelus septemfasciatus) embryos

文献类型: 外文期刊

作者: Tian, Yongsheng 1 ; Jiang, Jing 1 ; Song, Lini 1 ; Chen, Zhangfan 1 ; Zhai, Jieming; Liu, Jiangchun; Wang, Na 1 ; C 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R Chi

关键词: Cryopreservation;Vitrification;Epinephelus septemfasciatus;Embryos;Hatchability

期刊名称:CRYOBIOLOGY ( 影响因子:2.487; 五年影响因子:2.641 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The effects of cryopreservation and the vitrification solution on the embryo hatchability of the seven-band grouper Epinephelus septemfasciatus were evaluated in this study. Six small molecule cryoprotectants (PG, MeOH, Gly, DMF, DMSO and EG) and four macromolecular cryoprotectants (glucose, fructose, sucrose and trehalose) were used to determine the embryo toxicity levels. Results showed that the embryo survival rate was higher when the PM (24% PG + 16% MeOH):Gly ratios were 3:1 and 4:1. Further experiments showed that the embryo survival rates in PMG3S (35% PMG3 + 5% sucrose) and PMG3T (35% PMG3 + 5% trehalose) were relatively higher, which are 29.24 +/- 10.81% and 27.01 +/- 3.39%, respectively. When treated with PMG3S and PMG3T by using 5-step method, embryos at somite stage and tail-bud stage shrank in the first 6 min and gradually recovered in volume to the original. This indicated the successful permeation of the vitrification solutions into cells. Then, embryos at the embryoid body formation stage, the somite stage and the tail-bud stage were cryopreserved with PMG3S and PMG3T. In total, 82 floating embryos were obtained, 14 of which developed further, with 8 embryos at the tail-bud stage developing to the heartbeat stage, 4 embryos at the body formation stage development to the somite stage, and 2 embryos at the somite stage hatched to larval fish. (C) 2015 Elsevier Inc. All rights reserved.

  • 相关文献

[1]Sperm cryopreservation of sex-reversed seven-band grouper, Epinephelus septemfasciatus. Tian, Yongsheng,Qi, Wenshan,Jiang, Jing,Wang, Na,Wang, Di,Chen, Chao,Chen, Songlin,Qi, Wenshan,Jiang, Jing,Wang, Di,Zhai, Jieming.

[2]Interactive effects of incubation temperature and salinity on the early life stages of pacific cod Gadus macrocephalus. Bian, Xiaodong,Jin, Xianshi,Wan, Ruijing,Zhang, Xiumei,Gao, Tianxiang,Sakurai, Yasunari,Yamamoto, Jun. 2016

[3]Isolation and characterization of polymorphic microsatellite loci from a dinucleotide-enriched genomic library of seven-band grouper (Epinephelus septemfasciatus) and cross-species amplification. Zhao, Lili,Shao, Changwei,Liao, Xiaolin,Chen, Songlin,Zhao, Lili.

[4]EFFECTS OF VITRIFICATION PROTOCOL ON THE LACTATE DEHYDROGENASE AND TOTAL ATPASE ACTIVITIES OF CHINESE MITTEN CRAB Eriocheir sinensis EMBRYOS. Huang, Xiaorong,Feng, Guangpeng,Zhao, Feng,Liu, Jianyi,Zhang, Tao,Wang, Yu,Zhuang, Ping. 2016

[5]Salinity stress on embryos and early larval stages of the pomfret Pampus punctatissimus. Zhang, Dong,Zhang, Dong,Shi, Zhaohong,Huang, Xuxiong,Fu, Rongbin,Wang, Haiping,Luo, Haizhong,Chen, Bo,Liu, Minghai.

[6]Cryopreservation of sea perch (Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization. Ji, XS,Chen, SL,Tian, YS,Yu, GC,Sha, ZX,Xu, MY,Zhang, SC. 2004

[7]Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques. Li, Ping,Li, Zhi-Hua,Dzyuba, Boris,Hulak, Martin,Rodina, Marek,Linhart, Otomar,Li, Ping,Li, Zhi-Hua,Dzyuba, Boris. 2010

[8]Cryopreservation of the sperm of spotted halibut Verasper variegates (Pleuronectiformes, Pleuronectidae). Liu, XZ,Zhang, SC,Zhang, YZ,Xu, YJ. 2006

[9]Sperm of the giant grouper: cryopreservation, physiological and morphological analysis and application in hybridizations with red-spotted grouper. Tian, Yongsheng,Jiang, Jing,Wang, Na,Qi, Wenshan,Liang, You,Chen, Songlin,Jiang, Jing,Qi, Wenshan,Yang, Chuanjun,Zhai, Jieming,Li, Bo,Chen, Youming.

[10]Cryopreservation of common carp (Cyprinus carpio L.) sperm induces protein phosphorylation in tyrosine and threonine residues. Li, P.,Hulak, M.,Li, Z. H.,Psenicka, M.,Rodina, M.,Gela, D.,Linhart, O.,Li, P.,Li, Z. H.,Sulc, M..

[11]Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology. Zhang, YZ,Zhang, SC,Liu, XZ,Xu, YY,Wang, CL,Sawant, MS,Li, J,Chen, SL.

[12]Cryopreservation of sperm from Atlantic halibut (Hippoglossus hippoglossus, L.) for commercial application. Ding, Fuhong,Lei, Jilin,Lall, Santosh P.,Milley, Joyce E.,Li, Jun,Rommens, Melissa. 2011

[13]Cryopreservation of sperm from turbot (Scophthalmus maximus) and application to large-scale fertilization. Chen, SL,Ji, XS,Yu, GC,Tian, YS,Sha, ZX.

[14]EFFECTS OF CRYOPRESERVATION ON MOTILITY CHARACTERISTICS AND ENZYME ACTIVITY OF SPERM IN A CHINESE FISH, Nibea albiflora. Huang, Xiaorong,Zhuang, Ping,Zhang, Longzhen,Zhao, Feng,Liu, Jianyi,Feng, Guangpeng,Zhang, Tao. 2014

[15]Effect of hormone implantation on cryopreservation of Atlantic halibut (Hippoglossus hippoglossus L.) sperm. Milley, Joyce E.,Lall, Santosh P.,Ding, Fuhong,Lei, Jilin,Rommens, Melissa,Li, Jun.

[16]Long-term cryopreservation of sperm from Mandarin fish Siniperca chuatsi. Ding, Shuyan,Ge, Jiachun,Hao, Chen,Zhang, Mingsheng,Yan, Weihui,Xu, Zhiqiang,Pan, Jianlin,Chen, Songlin,Tian, Yongsheng,Huang, Yahong.

作者其他论文 更多>>