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Systematic identification of seven ribosomal protein genes in bighead carp and their expression in response to microcystin-LR

文献类型： 外文期刊

作者： Cai, Yan 1 ; Zhang, Chao 1 ; Hao, Le 3 ; Chen, Jun 2 ; Xie, Ping 2 ; Chen, Zhidong 1 ;

作者机构： 1.Changzhou Univ, Sch Petrolchem Engneering, Changzhou 213164, Peoples R China

2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China

3.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangdong Publ Lab Vet Publ Hlth, Guangzhou 510642, Guangdong, Peoples R China

4.South China Agr Univ, Guangzhou 510642, Guangdong, Peoples R China

关键词： Ribosomal protein;Microcystin-LR;Gene identification;Bighead carp

期刊名称：JOURNAL OF TOXICOLOGICAL SCIENCES （ 影响因子：2.196； 五年影响因子：2.177 ）

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收录情况： SCI

摘要： Microcystin-LR (MCLR) is one of the most toxic cyanotoxins produced in algal blooms. The toxic effects of MCLR on the expression of some organelles genes (mitochondrion, endoplasmic reticulum, and cytoskeleton etc) have been widely investigated, but little is known how it impacts on the expression of ribosomal genes. In this study we identified seven ribosomal protein genes RPS6, RPS12, RPS24, RPS27a, RPL12, RPL27 and RPL29 in bighead carp (Aristichthys nobilis), whose expression was regulated by MCLR. The amino acid sequences of those 7 genes shared more than 90% identity with corresponding sequences from zebrafish, and were well conserved throughout evolution. The 3D structure prediction showed that the structures of these ribosomal proteins were conserved, but had species specificity. Q-PCR analysis revealed that expression of seven genes changed dramatically at 3 hr, then went back to a moderate change-level at 24 hr in almost all tested tissues (liver, kidney, intestine, heart, spleen and gill) post MCLR injection, but in brain expression of the seven genes stayed same as the normal level. This study will help us to know not only about the evolution and functions of ribosomal proteins in anti-MCLR response in bighead carp, but also about the MCLR toxicity and its impact on aquaculture and human health.

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