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Development and Validation of an Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Simultaneous Determination of Four Type B Trichothecenes and Masked Deoxynivalenol in Various Feed Products

文献类型： 外文期刊

作者： Fan, Zhichen 1 ; Bai, Bing 1 ; Jin, Peng 1 ; Fan, Kai 1 ; Guo, Wenbo 1 ; Zhao, Zhihui 1 ; Han, Zheng 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, Shanghai 201403, Peoples R China

2.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China

关键词： type B trichothecenes;masked deoxynivalenol;HLB cartridges;feed products;ultra-high performance liquid chromatography-tandem mass spectrometry

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

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收录情况： SCI

摘要： A reliable and sensitive analytical method was developed for simultaneous determination of deoxynivalenol(DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), fusarenon X (FUS-X), and masked deoxynivalenol (deoxynivalenol-3-glucoside, D3G) in formula feed, concentrated feed, and premixed feed products. The method was based on an improved sample pretreatment with the commercially available HLB cartridges used for sample purification and enrichment followed by analysis using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Several key parameters including the extraction solvents, the positions of sample loading solvents, washing and elution solvents for HLB cartridges were carefully optimized to achieve optimal extraction and purification efficiencies. The established method was extensively validated by determining the linearity (R-2 >= 0.99), sensitivity (limit of quantification in the range of 0.08-4.85 mu g/kg), recovery (79.3%-108.1%), precision (Intra-day RSDs <= 13.5% and Inter-day RSDs <= 14.9%), and then was successfully applied to determine the four type B trichothecenes and D3G in a total of 31 feed samples. Among them, 26 were contaminated with various mycotoxins at the levels of 2.1-864.5 mu g/kg, and D3G has also been detected in 17 samples with the concentrations in the range of 2.1-34.8 mu g/kg, proving the established method to be a valuable tool for type B trichothecenes and masked DON monitoring in complex feed matrices.

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