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Effect of inhibin gene immunization on antibody production and reproductive performance in Partridge Shank hens

文献类型： 外文期刊

作者： Mao, Dagan 1 ; Bai, Wujiao 1 ; Hui, Fengming 1 ; Yang, Liguo 2 ; Cao, Shaoxian 3 ; Xu, Yinxue 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing, Jiangsu, Peoples R China

2.Huazhong Agr Univ, Coll Anim Sci & Technol, Wuhan, Hubei, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing, Jiangsu, Peoples R China

关键词： Hen;Inhibin;Gene immunization;Follicle development;Egg lay

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

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收录情况： SCI

摘要： To investigate the effect of inhibin gene immunization on antibody production and reproductive performance in broiler breeder females, Partridge Shank hens aged 380 days were immunized with inhibin recombinant plasmid pcISI. One hundred and twenty hens were randomly assigned to four groups and treated intramuscularly with 25, 75, or 125 mu g/300-mu L inhibin recombinant plasmid pcISI (T1 similar to T3) or 300-mu L saline as control (C), respectively. Booster immunization was given with the same dosage 20 days later. Blood and egg samples were collected to detect the antibody against inhibin by enzyme-linked immunosorbent assay and to evaluate egg performance. The ovaries were collected to classify the follicles and detect the FSH receptor (FSHR) messenger RNA (mRNA) expression by reverse transcription-PCR. The results showed that immunization against pcISI could elicit antibody against inhibin in both plasma and egg yolk compared with the control (P < 0.05), whereas booster immunization did not increase the antibody level in plasma. Vaccination promoted egg lay during the first 30 days after primary vaccination (P < 0.05) with no effect on egg quality and hatching rate. Immunization increased the amounts of dominant, small yellow and large white follicles in the ovary (P < 0.05). Reverse transcription-PCR results showed that immunization increased the FSHR mRNA in the large white follicles, whereas decreased the FSHR mRNA in the small yellow follicles (P < 0.05). In conclusion, inhibin vaccine pcISI can stimulate the production of antibody against inhibin as well as the follicle development and egg laying performance in Partridge Shank hens, which provides a good foundation for the application of inhibin DNA vaccine in avian production. (C) 2016 Elsevier Inc. All rights reserved.

  • 相关文献

[1]Immunization against inhibin improves in vivo and in vitro embryo production. Yan, Leyan,Li, Hui,Shi, Zhendan.

[2]Studies on enhancing embryo quantity and quality by immunization against inhibin in repeatedly superovulated Holstein heifers and the associated endocrine mechanisms. Liu, Y. P.,Chen, R. A.,Mao, X. B.,Wei, Y. M.,Yu, J. N.,Li, H.,Shi, Z. D..

[3]Transcriptome sequencing analysis of porcine granulosa cells treated with an anti-inhibin antibody. Lei, Mingming,Cai, Liuping,Li, Hui,Chen, Zhen,Shi, Zhendan. 2017

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