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Identification of novel alleles induced by EMS-mutagenesis in key genes of kernel hardness and starch biosynthesis in wheat by TILLING

文献类型： 外文期刊

作者： Li, Wenjie 1 ; Guo, Huijun 2 ; Wang, Yongbin; Xie, Yongdun 2 ; Zhao, Linshu 2 ; Gu, Jiayu 2 ; Zhao, Shirong 3 ; Zhao, 1 ;

作者机构： 1.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050024, Peoples R China

2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Natl Ctr Space Mutagenesis Crop Improvement, Beijing 100081, Peoples R China

3.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Faci

关键词： Wheat;TILLING;Point mutation;Kernel hardness;Starch synthesis

期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）

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收录情况： SCI

摘要： To identify novel allelic variations in key genes of wheat quality, the present study used the targeting induced local lesions in genomes platform to detect point mutations in target genes. The wheat variety Long-fumai 17 was treated by the mutagen ethyl methanesulfonate to produce a bulk M-2 generation, and the population included 1122 plants. A total length of 3906.80 kb nucleotides was analyzed, and the average mutation density was 1/244.17 kb. The identified mutations included G>A substitutions (43.75%), C>T substitutions (31.25%), A insertions (12.50%), T insertions (6.25%), and deletions (6.25%). These point mutations led to changes in amino acids and thus the encoded protein sequences, ultimately producing 18.75% of missense mutations, 12.50% of frame shift mutations, 6.25% of nonsense mutations, 25.00% of silent mutations and 37.50% of non-coding region mutations. In the kernel hardness gene Pinb and 3 starch synthesis genes waxy, Agp2 and SSIIa-A, we detected 16 different point mutations in 25 mutant lines. The Pinb gene harbored two missense mutations and a nonsense mutation; the C>T missense mutation resulted in a novel allele, this novel allele and the nonsense mutation alerted protein 3D structure; the waxy gene presented missense and frame shift mutations; the Agp2 gene carried a missense mutation; the SSIIa-A incurred a missense mutation and a frame shift mutation that resulted in premature protein termination. All the frame shift mutations, nonsense mutations and the Pinb novel allele resulted in allelic variation of their corresponding genes, which in turn affected their gene functions. The identified mutant lines can be used as intermediate materials in wheat quality improvement schemes.

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