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20-Hydroxyecdysone activates PGRP-SA mediated immune response in Locusta migratoria

文献类型： 外文期刊

作者： Han, Pengfei 1 ; Han, Jiao 1 ; Fan, Jigiao 2 ; Zhang, Min 1 ; Ma, Enbo 1 ; Li, Sheng; Fan, Renjun 2 ; Zhang, Jianzhen 1 ;

作者机构： 1.Shanxi Univ, Inst Appl Biol, Taiyuan 030006, Peoples R China

2.Shanxi Acad Agr Sci, Inst Plant Protect, Taiyuan 030000, Peoples R China

3.Shanxi Key Lab Integrated Pest Management Agr, Yangling 030006, Shaanxi, Peoples R China

4.Shanxi Key Lab Integrated

关键词： Locusta migratoria;20-Hydroxyecdysone;PGRP;Immunity

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

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收录情况： SCI

摘要： 20-hydroxyecdysone (20E) has been implicated in regulating the immune response in insects. Conflicting conclusions on 20E regulating immunity have been reported in model holometabolous species. However, in hemimetabolous insects, the role of 20E as an immune-suppressor or activator and the mechanism remains unclear. The migratory locust Locusta migratoria is a representative member of hemimetabolous insects. Here, digital gene expression (DGE) profiles of Locusta migratoria treated with 20E were analyzed. Pattern recognition receptors [peptidoglycan recognition protein (PGRP-SA), PGRP-LE, and gram-negative binding protein (GNBP3)] and antimicrobial peptides (defensin, diptericin, and i-type Iysozyme) were significantly induced by 20E in fat body. These immune-related genes significantly increased their mRNA levels during the high-20E stage. Antibacterial activities in plasma were enhanced after 20E injection and during the high-20E developmental stage. Conversely, when 20E signal was suppressed by RNAi of EcR (ecdysone receptor), the expression levels of these genes and antibacterial activities failed to be increased by 20E injection and during the high-20E developmental stage, and the mortality increased after being infected by entomogenous fungus. The knockdown of PGRP-SA inhibited the expression level of defensin, diptericin and i-type lysozyme in fat body and reduced antibacterial activities in plasma. 20E injection could not significantly induce the expression of antimicrobial peptides after RNAi of PGRP-SA. These results demonstrated that 20E enhanced the immune response by activating PGRP-SA in L migratoria. (C) 2017 Elsevier Ltd. All rights reserved.

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