您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Establishment of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Soybean Allergen beta-Conglycinin

文献类型： 外文期刊

作者： Wang, Yao 1 ; Li, Zhaozhou 1 ; Pei, Yafeng 1 ; Li, Qingmei 2 ; Sun, Yaning 2 ; Yang, Jifei 2 ; Yang, Yanyan 2 ; Zhi, Yuba 1 ;

作者机构： 1.Henan Univ Sci & Technol, Coll Food & Bioengn, Luoyang 471023, Henan, Peoples R China

2.Henan Acad Agr Sci, Henan Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China

3.Henan Acad Agr Sci, Henan Key Lab Anim Immunol

关键词： beta-Conglycinin;Immunochromatographic strip;Colloidal gold;Immunoassay

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.366； 五年影响因子：3.07 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A rapid lateral flow colloidal gold immunoassay strip was established in a sandwich format with the colloidal gold-labelled mouse anti-beta-conglycinin monoclonal antibody (mAb) and the rabbit anti-beta-conglycinin polyclonal antibody (pAb) in order to specifically identify a soybean allergen, beta-conglycinin. Soybean and soy derivatives have become ubiquitous in many vegetarian and meat-based food products, and as a result, dietary avoidance has become difficult. Therefore, soybean avoidance is challenging for individuals with soybean allergy. In our study, the selected mAb showed high affinity to the target and no cross-reactivity with other soybean allergens. Based on the sandwich immunoassay principle, the rabbit pAb was applied in the test line with high titre. The assay could be accomplished within 10 min without sophisticated procedures. The limit of detection of the test strip was calculated at 1.66 mg/kg in powdered milk using an optical density scanner that measures relative optical density. The assay showed high specificity for beta-conglycinin, with no cross-reactions with other food allergens. The efficacy of the lateral flow test strip to detect beta-conglycinin in powdered milk samples ranged between 80.8 and 89.2% with relative standard deviations of less than 7.2%. Therefore, the test strip is useful as a rapid and reliable detection method for beta-conglycinin in powdered milk. In addition, this assay is suitable for monitoring beta-conglycinin in other processed foods when samples are appropriately extracted.

  • 相关文献

[1]Rapid and Sensitive Detection of the Food Allergen Glycinin in Powdered Milk Using a Lateral Flow Colloidal Gold Immunoassay Strip Test. Wang, Yao,Li, Zhixi,Deng, Ruiguang,Zhang, Gaiping,Li, Qingmei,Yang, Jifei,Sun, Yaning,Hu, Xiaofei,Zhang, Gaiping.

[2]Development of a lateral flow immunoassay strip for screening of sulfamonomethoxine residues. Zhang, G.,Wang, X.,Bao, Y.,Wang, Z.,Zhang, G.,Wang, X.,Yang, Y.,Luo, J.,Li, Q.,Guo, J.,Yang, J.,Xing, G.,Chai, S.,Shi, T.,Liu, Q.,Zhi, A.,Qu, M.. 2008

[3]Development of an Immunochromatographic Strip Test for the Rapid Detection of Zearalenone in Corn. Sun, Yaning,Hu, Xiaofei,Yang, Jifei,Wang, Fangyu,Wang, Yao,Deng, Ruiguang,Zhang, Gaiping,Sun, Yaning,Zhang, Yong,Zhang, Gaiping.

[4]Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Olaquindox Residues. Song, Chunmei,Liu, Qingtang,Zhi, Aimin,Yang, Jifei,Zhi, Yubao,Li, Qingmei,Hu, Xiaofei,Deng, Ruiguang,Zhang, Gaiping,Song, Chunmei,Casas, Justin,Tang, Liang.

[5]Development of an immunochromatographic test strip for simultaneous qualitative and quantitative detection of ochratoxin A and zearalenone in cereal. Sun, Yaning,Xing, Guangxu,Yang, Jifei,Wang, Fangyu,Deng, Ruiguang,Zhang, Gaiping,Hu, Xiaofei,Sun, Yaning,Zhang, Yong,Zhang, Gaiping.

[6]Development of a colloidal gold-based strip test for the detection of chlorothalonil residues in cucumber. Yang, Xingdong,Zeng, Xianyin,Yang, Xingdong,Zhang, Gaiping,Wang, Fangyu,Wang, Yinbiao,Hu, Xiaofei,Li, Qingmei,Jia, Guochao,Liu, Zhen,Wang, Yao,Deng, Ruiguang,Zhang, Gaiping.

[7]Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Enrofloxacin Residues. Zhao, Yinli,Zhang, Gaiping,Liu, Qingtang,Teng, Man,Yang, Jifei,Zhao, Yinli,Wang, Jianhua.

[8]Development of an immunochromatographic strip for the detection of antibodies against Porcine circovirus-2. Jin, Qianyue,Wang, Yinbiao,Chen, Wen,Jin, Qianyue,Yang, Jifei,Lu, Qingxia,Guo, Junqing,Deng, Ruiguang,Wang, Yinbiao,Wang, Shihong,Wang, Shufen,Chen, Wen,Zhi, Yubao,Wang, Li,Yang, Suzhen,Zhang, Gaiping,Wang, Shufen,Wang, Shufen. 2012

[9]Development of a peptide-based immunochromatographic strip for differentiation of serotype O Foot-and-mouth disease virus-infected pigs from vaccinated pigs. Yang, Suzhen,Yang, Jifei,Zhang, Gaiping,Qiao, Songlin,Zhao, Dong,Li, Xuewu,Deng, Ruiguang,Zhi, Aimin,You, Leiming,Chai, Sujun,Teng, Man,Yang, Suzhen,Wang, Xuannian. 2010

[10]A lateral flow assay for the determination of human tetanus antibody in whole blood by using gold nanoparticle labeled tetanus antigen. Liu, Juanzu,Li, Zhaohui,Meng, Hongmin,Zhang, Lin,Qu, Lingbo,Wang, Junyan,Wang, Hongqi,Li, Zhaohui,Qu, Lingbo. 2018

[11]Development of an immunochromatographic strip for the detection of antibodies against foot-and-mouth disease virus serotype O. Yang, Suzhen,Yang, Jifei,Zhang, Gaiping,Qiao, Songlin,Zhao, Dong,Zhi, Yubao,Li, Xuewu,Xing, Guangxu,Luo, Jun,Fan, Jianming,Bao, Dengke,Yang, Suzhen,Qiao, Songlin,Bao, Dengke,Wang, Xuannian. 2010

[12]Development and evaluation of an immunochromatographic strip for trichinellosis detection. Zhang, GP,Guo, JQ,Wang, XN,Yang, JX,Yang, YY,Li, QM,Li, XW,Deng, RG,Xiao, ZJ,Yang, JF,Xing, GX,Zhao, D. 2006

[13]Development of an immunochromatographic strip for antibody detection of pseudorabies virus in swine. Li, Xiujie,Sun, Yaning,Yang, Suzhen,Wang, Yinbiao,Yang, Jifei,Liu, Yunchao,Jin, Qianyue,Li, Xuewu,Guo, Chengliu,Zhang, Gaiping,Zhang, Gaiping,Li, Xiujie,Yang, Suzhen,Zhang, Gaiping.

[14]Development of an immunochromatographic strip for rapid detection of antibodies against classical swine fever virus. Li, Xuewu,Wang, Li,Shi, Xibao,Zhao, Dong,Yang, Jifei,Yang, Suzhen,Zhang, Gaiping.

[15]Development of an immunochromatographic lateral flow test strip for detection of beta-adrenergic agonist Clenbuterol residues. Zhang, GP,Wang, XN,Yang, JF,Yang, YY,Xing, GX,Li, QM,Zhao, D,Chai, SJ,Guo, JQ. 2006

[16]Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples. Yang, Xing-dong,Wang, Fang-yu,Zhang, Gai-ping,Yang, Xing-dong,Zeng, Xian-yin,Song, Chun-mei,Wu, Shu-yan,Zhang, Gai-ping.

[17]Development of a one-step strip test for the diagnosis of chicken infectious bursal disease. Zhang, GP,Li, QM,Yan, YY,Guo, JQ,Li, XW,Deng, RG,Xiao, ZJ,Xing, GX,Yang, JF,Zhao, D,Cai, SJ,Zang, WM. 2005

[18]Development of a lateral-flow immunochromatographic test device for the rapid detection of difloxacin residues. Zhi, Ai-min,Li, Bin-bin,Liu, Qing-tang,Hu, Xiao-fei,Zhao, Dong,Hou, Yu-ze,Deng, Rui-guang,Zhang, Gai-ping,Li, Bin-bin,Chai, Shu-Jun. 2010

[19]Colloidal gold-McAb probe-based rapid immunoassay strip for simultaneous detection of fumonisins in maize. Yao, Jingjing,Sun, Yaning,Li, Qingmei,Wang, Fangyu,Teng, Man,Yang, Yanyan,Deng, Ruiguang,Hu, Xiaofei.

[20]Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples. Hua, Xiude,Qian, Guoliang,Hu, Baishi,Fan, Jiaqin,Qin, Na,Li, Gang,Wang, Yuyan,Liu, Fengquan,Yang, Jifei.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台