您好，欢迎访问湖北省农业科学院 机构知识库！
湖北省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Comparative proteomic analysis of alfalfa revealed new salt and drought stress-related factors involved in seed germination

文献类型： 外文期刊

作者： Ma, Qiaoli 1 ; Kang, Junmei 1 ; Long, Ruicai 2 ; Zhang, Tiejun 2 ; Xiong, Junbo 4 ; Zhang, Kun 1 ; Wang, Tenghua 1 ; Yang 1 ;

作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, Beijing 100193, Peoples R China

2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

3.Ningxia Univ, Sch Chem & Chem Engn, Yinchuan 750021, Peoples R China

4.Hubei Acad Agr Sci, Inst Anim Sci, Wuhan 430064, Hubei, Peoples R China

关键词： Alfalfa;Salt stress;Drought stress;Seed germination;Proteomics

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Salinity and drought are two major environmental factors that limit the growth and yield of many forage crops in semi-arid and arid regions. Alfalfa (Medicago sativa L.) is one of the most important forage crops in many countries. We aim to investigate the molecular mechanisms of alfalfa in response to salt and drought stresses in this study. Physiological and proteomic analyses were applied to examine the Zhongmu NO.3 alfalfa seed germination stage with 200 mM NaCl and 180 g center dot L-1 polyethylene glycol (PEG) treatments. The germination ability of the seed and the accumulation of osmotic solutes were quite different between the NaCl and PEG treatments. More than 800 protein spots were detected by proteomics technology on two-dimensional electrophoresis (2-DE) gels. The abundance of twenty-eight proteins were decreased or increased after salt and drought stress. Seventeen of these proteins were identified and classified into six functional categories through mass spectrometry (MS). The six groups involved in salt- and PEG-mediated stress included defense response, energy metabolism, protein synthesis and degradation, oxidative stress, carbohydrate metabolism-associated proteins, and unknown proteins. We discovered that some proteins related to carbohydrate metabolism and energy production increased in abundance under salt- and PEG-mediated drought stress. This demonstrates a common mechanism of energy consumption during abiotic stresses. Further study of these proteins with unknown function will provide insights into the molecular mechanisms of abiotic stress and the discovery of new candidate markers.

  • 相关文献

[1]Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings. Ma Qiao-li,Sun Yan,Kang Jun-mei,Long Rui-cai,Cui Yan-jun,Zhang Tie-jun,Xiong Jun-bo,Yang Qing-chuan,Ma Qiao-li,Xiong Jun-bo. 2016

[2]Proteomics analysis identified a DRT protein involved in arsenic resistance in Populus. Liu, Yanli,Damaris, Rebecca Njeri,Yang, Pingfang,Liu, Yanli,Yang, Pingfang,Yang, Pingfang.

[3]Effective protein extraction protocol for proteomics studies of Jerusalem artichoke leaves. Shen, Shihua,Zhang, Meide.

[4]SnRK2 Homologs in Gossypium and GhSnRK2.6 Improved Salt Tolerance in Transgenic Upland Cotton and Arabidopsis. Su, Ying,Zhen, Junbo,Zhang, Xi,Chen, Zhiwen,Li, Le,Hua, Jinping,Wang, Yumei,Zhen, Junbo,Huang, Yi.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖北省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台