Protective efficacy of cationic-PLGA microspheres loaded with DNA vaccine encoding the sip gene of Streptococcus agalactiae in tilapia
文献类型： 外文期刊
作者： Ma, Yan-ping 1 ; Ke, Hao 1 ; Liang, Zhi-ling 1 ; Ma, Jiang-yao 1 ; Hao, Le 1 ; Liu, Zhen-xing 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangdong Open Lab Vet Publ Hlth, Guangdong Prov Key Lab Livestock Dis Prevent, Guangzhou 510640, Guangdong, Peoples R China
关键词： Tilapia streptococcus agalactiae;Cationic-PLGA microspheres;Oral vaccine;Immunogenicity analysis
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
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收录情况： SCI
摘要： Streptococcus agalactiae (S. agalactiae) is an important fish pathogen, which has received more attention in the past decade due to the increasing economic losses in the tilapia industry worldwide. As existing effective vaccines of S. agalactiae in fish have obvious disadvantage, to select immunoprotective antigens and package materials would undoubtedly contribute to the development of novel oral vaccines. In the present study, surface immunogenic protein (sip) was selected from the S. agalactiae serovar I a genomes as immunogenic protein in DNA vaccine form with cationic chitosan and biodegradable and biocompatible PLGA. The pcSip plasmid in cationic-PLGA was successfully expressed in tissues of immunized tilapia and the immunogenicity was assessed in tilapia challenge model. A significant increase was observed in the cytokine levels of IL-1 beta, TNF-alpha, CC1, CC2 in spleen and kidney tissues. Furthermore, immunized tilapia conferred different levels of protection against challenge with a lethal dose of highly virulent serovar I a S. agalactiae. Our results indicated that the pcSip plasmid in cationic-PLGA induced high level of antibodies and protection against S. agalactiae infection, could be effective oral DNA vaccine candidates. (C) 2017 Elsevier Ltd. All rights reserved.
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