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Novel fluoroimmunoassays for detecting ochratoxin A using CdTe quantum dots

文献类型： 外文期刊

作者： Yao, Jingjing 1 ; Wang, Fangyu 1 ; Han, Junling 2 ; Sun, Yaning 1 ; Xing, Guangxu 1 ; Deng, Ruiguang 1 ; Hu, Xiaofei 1 ;

作者机构： 1.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Henan Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

2.Xinxiang Med Univ, Affiliated Hosp 1, Xinxiang 453100, Peoples R China

3.Henan Agr Univ, Zhengzhou 450002, Peoples R China

关键词： CdTe quantum dots;ochratoxin A;fluoroimmunoassay;FLISA

期刊名称：JOURNAL OF BIOPHOTONICS （ 影响因子：3.207； 五年影响因子：3.403 ）

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收录情况： SCI

摘要： Novel direct and indirect competitive fluorescence-linked immunosorbent assays (cFLISA and icFLISA) for detection of ochratoxin A (OTA) were described using CdTe quantum dots (QDs) as fluorescent label. CdTe QDs were successfully synthesized, which had an emission wavelength of 615 nm. The high purity monoclonal antibody against OTA was prepared through cell thawing and the octylic acid-ammonium sulfate method. The OTA MAbs were successfully coupled with CdTe QDs, and which also retained the original biological activity. The 50% inhibition values (IC50) of the cFLISA and icFLISA were 0.630 ng/mL, 0.234 ng/mL, the limits of detection (LOD) were 7.06 x 10(-3) and 4.15 x 10(-3) ng/mL, and detection ranges were 7.06 x 10(-3) - 18.34 ng/mL and 4.15 x 10(-3) - 4.88 ng/mL, in-order. The recoveries were 96.0-104.7% along with coefficients of variation (CVs) below 10%. The FLISA provided novel method for determination of OTA and the potential of MAb-CdTe QDs for the establishment of fluorescent immunochromatographic test strip.

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[1]Development of an immunochromatographic test strip for simultaneous qualitative and quantitative detection of ochratoxin A and zearalenone in cereal. Sun, Yaning,Xing, Guangxu,Yang, Jifei,Wang, Fangyu,Deng, Ruiguang,Zhang, Gaiping,Hu, Xiaofei,Sun, Yaning,Zhang, Yong,Zhang, Gaiping.

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