文献类型： 外文期刊
作者： Yao, Jingjing 1 ; Wang, Fangyu 1 ; Han, Junling 2 ; Sun, Yaning 1 ; Xing, Guangxu 1 ; Deng, Ruiguang 1 ; Hu, Xiaofei 1 ;
作者机构： 1.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Henan Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
2.Xinxiang Med Univ, Affiliated Hosp 1, Xinxiang 453100, Peoples R China
3.Henan Agr Univ, Zhengzhou 450002, Peoples R China
关键词： CdTe quantum dots;ochratoxin A;fluoroimmunoassay;FLISA
期刊名称：JOURNAL OF BIOPHOTONICS （ 影响因子：3.207； 五年影响因子：3.403 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Novel direct and indirect competitive fluorescence-linked immunosorbent assays (cFLISA and icFLISA) for detection of ochratoxin A (OTA) were described using CdTe quantum dots (QDs) as fluorescent label. CdTe QDs were successfully synthesized, which had an emission wavelength of 615 nm. The high purity monoclonal antibody against OTA was prepared through cell thawing and the octylic acid-ammonium sulfate method. The OTA MAbs were successfully coupled with CdTe QDs, and which also retained the original biological activity. The 50% inhibition values (IC50) of the cFLISA and icFLISA were 0.630 ng/mL, 0.234 ng/mL, the limits of detection (LOD) were 7.06 x 10(-3) and 4.15 x 10(-3) ng/mL, and detection ranges were 7.06 x 10(-3) - 18.34 ng/mL and 4.15 x 10(-3) - 4.88 ng/mL, in-order. The recoveries were 96.0-104.7% along with coefficients of variation (CVs) below 10%. The FLISA provided novel method for determination of OTA and the potential of MAb-CdTe QDs for the establishment of fluorescent immunochromatographic test strip.
- 相关文献
作者其他论文 更多>>
-
Enhancing humoral and mucosal immune response of PED vaccine candidate by fusing S1 protein to nanoparticle multimerization
作者：Li, Minghui;Sun, Xueke;Wang, Siqiao;Wang, Yanan;Wang, Yue;Zhang, Gaiping;Li, Minghui;Sun, Xueke;Chen, Yilan;Wang, Siqiao;Li, Qin;Wang, Yanan;Wang, Yue;Li, Ruiqi;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping
关键词：PEDV; S1 protein; Nanoparticle; Subunit vaccine; Mucosal immunity
-
Pseudorabies virus tegument protein US2 antagonizes antiviral innate immunity by targeting cGAS-STING signaling pathway
作者：Kong, Zhengjie;Zhang, Yifeng;Guan, Kaifeng;Yao, Wanzi;Kang, Yu;Lu, Xinyi;Zhang, Gaiping;Chen, Xing;Gong, Lele;Wang, Lele;Zhang, Yuhang;Du, Yongkun;Sun, Aijun;Zhuang, Guoqing;Wan, Bo;Zhang, Gaiping;Zhao, Jianguo;Zhang, Gaiping;Zhang, Gaiping
关键词：pseudorabies virus; cGAS-STING; tegument protein US2; TRIM21; immune invasion
-
IFI16 Positively Regulates RIG-I-Mediated Type I Interferon Production in a STING-Independent Manner
作者：Shi, Xibao;Wei, Menglu;Feng, Yuwen;Yang, Yuanhao;Zhang, Xiaozhuan;Chen, Hao;Xing, Yuqi;Wang, Keqi;Wang, Wensheng;Wang, Li;Zhang, Gaiping;Wang, Aiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Shi, Xibao;Zhang, Gaiping
关键词：IFN-gamma-inducible protein 16; interferon gene-stimulating protein; retinoic acid-inducible gene I; type I interferon
-
In Vitro Protective Effect of Pea-Derived Peptides (PPs) via the Keap1/Nrf2 Signaling Pathway on Alpha-Gliadin-Sensitizing Peptide Induced Cacao-2 Cells
作者：Gao, Bing;Yan, Fang;Wang, Chunfeng;Cui, Chenxu;Sun, Xuefeng;Wang, Fangyu;Li, Ning
关键词：antioxidant enzymes; celiac disease; free radicals scavenging; alpha-gliadin peptide; Keap1/Nrf2 pathway
-
A universal design of restructured dimer antigens: Development of a superior vaccine against the paramyxovirus in transgenic rice
作者：Ma, Fanshu;Zhang, Longxian;Zhang, Erqin;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Xu, Qianru;Yang, Daichang;Li, Qingmei;Guo, Junqing;Li, Rui;Wang, Li;Wang, Yanan;Zhao, Xiangyue;Chai, Shujun;Yin, Heng;Li, Kunpeng;Rao, Zihe;Rao, Zihe;Rao, Zihe
关键词：structural vaccine; transgenic rice; BCR; paramyxovirus
-
Double-layered N-S1 protein nanoparticle immunization elicits robust cellular immune and broad antibody responses against SARS-CoV-2
作者：Li, Ruiqi;Chang, Zejie;Cheng, Anchun;Zhang, Gaiping;Li, Ruiqi;Zhang, Gaiping;Li, Ruiqi;Zhang, Gaiping;Li, Ruiqi;Chang, Zejie;Wang, Yanan;Li, Minghui;Chen, Yilan;Fan, Lu;Wang, Siqiao;Sun, Xueke;Liu, Siyuan;Zhang, Gaiping;Chang, Zejie;Wang, Yanan;Li, Minghui;Sun, Xueke;Liu, Siyuan;Zhang, Gaiping;Liu, Hongliang;Ding, Peiyang;Zhang, Gaiping
关键词：COVID-19; Coronavirus; SARS-CoV-2; Nanoparticle; Subunit vaccine; Variants
-
Porcine reproductive and respiratory syndrome virus triggers Golgi apparatus fragmentation-mediated autophagy to facilitate viral self-replication
作者：Zhao, Shuang-shuang;Qian, Qisheng;Zhang, Gaiping;Zhao, Shuang-shuang;Qian, Qisheng;Chen, Xin-xin;Lu, Qingxia;Xing, Guangxu;Qiao, Songlin;Li, Rui;Zhang, Gaiping;Zhang, Gaiping
关键词：autophagy; GA fragmentation; GRASP65; Nsp2; PRRSV; RAB2; ULK1
