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Gene characteristics, immune and stress responses of PmPrx1 in black tiger shrimp (Penaeus monodon): Insights from exposure to pathogenic bacteria and toxic environmental stressors

文献类型： 外文期刊

作者： Bu, Ruiqian 1 ; Wang, Pengfei 1 ; Zhao, Chao 1 ; Bao, Weiyang; Qiu, Lihua 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, 231 Xingangxi Rd, Guangzhou 510300, Guangdong, Peoples R China

2.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai, Peoples R China

3.Minist Agr, Key Lab South China Sea Fishery Resources Exploit, Guangzhou, Guangdong, Peoples R China

4.Minist Agr, Key Lab

关键词： Penaeus monodon;PmPrx1;Bacteria stimulation;Environmental acute stresses;mRNA expression;Antioxidant & antibacterial properties

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN：

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收录情况： SCI

摘要： Peroxiredoxins (Prxs) are ubiquitous, multifunctional and evolutionarily conserved enzymes that can protect cells from oxidative damage caused by ROS and play a vital role in immune responses. Here, a full-length Prx1 cDNA sequence (PmPrx1) was isolated from Penaeus monodon. The PmPrx1 cDNA was 951 base pairs (bp), encoding 198 amino acid polypeptides. The results of qRT-PCR showed that the PmPrx1 mRNA was ubiquitously expressed in all tissues tested and had a comparatively high expression level in immune-associated tissues (gill, hepatopancreas). To explore the immune and anti-stress roles of PmPrx1, the gills and hepatopancreas were chosen as target tissues in Penaeus monodon and were challenged with bacteria (Vibrio harveyi and Streptococcus agalactiae) and toxic environmental stresses. To further clarify the immune function of PmPrx1 after bacterial challenge, the recombinant PmPrx1 protein was acquired using a prokaryotic expression method. The antioxidant activity of the recombinant PmPrx1 was assessed by the catalyzing hydrogen peroxide assay method, and the results showed obvious antioxidant activity in a dose-dependent and temperature-dependent manner. The antimicrobial activity of purified PmPrx1 protein was evaluated and further studied in vitro relying on a bacterial growth inhibition test which was conducted in both liquid and solid cultures. Furthermore, E. coli transferred with pRSET-PmPrx1 was dramatically protected in response to metal toxicity and H2O2 oxidative stress. In summary, this study provides useful information about the role of the Prx1 gene in defense against a variety of toxic factors in shrimps that help to further clarify the functional mechanism of Prx. (C) 2017 Elsevier Ltd. All rights reserved.

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