Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a truncated version of -glucosidase from Aspergillus niger
文献类型： 外文期刊
作者： Li, Zhenjun 1 ; Han, Hongjuan 1 ; Wang, Bo 1 ; Gao, Jianjie 1 ; Zhu, Bo 1 ; Peng, Rihe 1 ; Yao, Quanhong 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai 201106, Peoples R China
关键词： 2-O--d-glucopyranosyl-l-ascorbic acid;-glucosidase;Aspergillus niger;high performance liquid chromatography;transglucosylation
期刊名称：JOURNAL OF FOOD BIOCHEMISTRY （ 影响因子：2.72； 五年影响因子：2.543 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： A truncated version of -glucosidase gene (AglA) from Aspergillus niger, named as AglA-t in this study, was expressed in Pichia pastoris, and the enzyme was purified and characterized. Phylogenetic analysis demonstrated that AglA was most close to the -glucosidase from Aureobasidium pullulans. In our research, we found that AglA-t could promote the formation of 2-O--d-glucopyranosyl-l-ascorbic acid (AA-2G) under optimized conditions. The major product was detected by high performance liquid chromatography, and confirmed to be by LC-MS analysis. The optimum pH and temperature for the transglucosylation reaction were 6.0 degrees C and 40 degrees C, respectively. The yield of AA-2G was significantly enhanced by metal ions Fe3+, Zn2+, Mg2+, and Al3+, and organic solvent isopropanol.
- 相关文献
作者其他论文 更多>>
-
Synthesis of betanin by expression of the core betalain biosynthetic pathway in carrot
作者：Wang, Bo;Wang, Yahui;Deng, Yuanjie;Xiong, Aisheng;Wang, Bo;Yao, Quanhong
关键词：Daucus carota L.; Betanin; Biosynthesis; Metabolic engineering; Bioforti fication
-
The complete degradation of 1,2-dichloroethane in Escherichia coli by metabolic engineering
作者：Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong;Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong
关键词：Bioremediation; Engineered bacteria; Environmental pollution; Metabolic engineering; Synthetic biology
-
Ectopic expression of an Old Yellow Enzyme (OYE3) gene from Saccharomyces cerevisiae increases the tolerance and phytoremediation of 2-nitroaniline in rice
作者：Li, Zhenjun;Gao, Jianjie;Wang, Bo;Zhang, Hao;Tian, Yongsheng;Peng, Rihe;Yao, Quanhong
关键词：2-nitroaniline; Old Yellow Enzyme; Phytoremediation; Reactive oxygen species; Saccharomyces cerevisiae
-
Biosynthesis of melatonin from
l -tryptophan by an engineered microbial cell factory作者：Wang, Lijuan;Deng, Yongdong;Gao, Jianjie;Wang, Bo;Han, Hongjuan;Li, Zhenjun;Zhang, Wenhui;Wang, Yu;Fu, Xiaoyan;Peng, Rihe;Yao, Quanhong;Tian, Yongsheng;Xu, Jing;Wang, Lijuan;Deng, Yongdong;Peng, Rihe;Yao, Quanhong;Tian, Yongsheng;Xu, Jing
关键词：Biosynthesis; Melatonin;
l -Tryptophan (l -Trp); Escherichia coli; Metabolic engineering -
Metabolic engineering of Escherichia coli for 2,4-dinitrotoluene degradation
作者：Zhang, Wen-Hui;Deng, Yong-Dong;Zuo, Zhi-Hao;Tian, Yong-Sheng;Xu, Jing;Wang, Bo;Wang, Li-Juan;Han, Hong-Juan;Li, Zhen-Jun;Wang, Yu;Yao, Quan-Hong;Gao, Jian-Jie;Fu, Xiao-Yan;Peng, Ri-He;Zhang, Wen-Hui;Deng, Yong-Dong;Zuo, Zhi-Hao;Tian, Yong-Sheng;Xu, Jing;Wang, Bo;Wang, Li-Juan;Han, Hong-Juan;Li, Zhen-Jun;Wang, Yu;Yao, Quan-Hong;Gao, Jian-Jie;Fu, Xiao-Yan;Peng, Ri-He;Chen, Zhi-Feng
关键词：4-Dinitrotoluene; Re-synthesize gene; Complete degradation; Bioremediation; Escherichia coli
-
Creation of Environmentally Friendly Super "Dinitrotoluene Scavenger" Plants
作者：Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Zhu, Bo
关键词：2,4-dinitrotoluene (2,4-DNT); carbon neutrality; complete degradation; rapid phytoremediation; sustainable development goals
-
Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot
作者：Wang, Bo;Wang, Ya-Hui;Deng, Yuan-Jie;Xiong, Ai-Sheng;Wang, Bo;Yao, Quan-Hong
关键词：Genetically modified carrot; Betanin; Unintended effects; Photosynthesis; Transcriptome; Metabolomics
