Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a truncated version of -glucosidase from Aspergillus niger
文献类型： 外文期刊
作者： Li, Zhenjun 1 ; Han, Hongjuan 1 ; Wang, Bo 1 ; Gao, Jianjie 1 ; Zhu, Bo 1 ; Peng, Rihe 1 ; Yao, Quanhong 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai 201106, Peoples R China
关键词： 2-O--d-glucopyranosyl-l-ascorbic acid;-glucosidase;Aspergillus niger;high performance liquid chromatography;transglucosylation
期刊名称：JOURNAL OF FOOD BIOCHEMISTRY （ 影响因子：2.72； 五年影响因子：2.543 ）
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收录情况： SCI
摘要： A truncated version of -glucosidase gene (AglA) from Aspergillus niger, named as AglA-t in this study, was expressed in Pichia pastoris, and the enzyme was purified and characterized. Phylogenetic analysis demonstrated that AglA was most close to the -glucosidase from Aureobasidium pullulans. In our research, we found that AglA-t could promote the formation of 2-O--d-glucopyranosyl-l-ascorbic acid (AA-2G) under optimized conditions. The major product was detected by high performance liquid chromatography, and confirmed to be by LC-MS analysis. The optimum pH and temperature for the transglucosylation reaction were 6.0 degrees C and 40 degrees C, respectively. The yield of AA-2G was significantly enhanced by metal ions Fe3+, Zn2+, Mg2+, and Al3+, and organic solvent isopropanol.
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